Abstract
Peanut allergic consumers rely on food package labels to avoid foods containing peanut. The inadvertent presence of peanut in foods due to cross-contact can be fatal if ingestion of such food leads to an allergic reaction. Analytical methods are available to detect undeclared peanut in foods. However, depending on the type of food matrix and food processing parameters, method performance can be adversely affected due to reduction in the extraction efficiency of peanut proteins. Temperature and probe sonication were used as a preincubation treatment for peanut flour slurries to assess their effect on the total peanut protein solubility from raw, light-roasted, and dark-roasted peanut flours. The effect of these treatments on the immunoreactivity of peanut allergens (Ara h 1, Ara h 2, Ara h 3, and Ara h 6) was determined by an indirect enzyme-linked immunosorbent assay using antibodies raised against these individual peanut proteins. Preincubation at 50 °C did not significantly improve the peanut protein solubility, whereas an increase in protein solubility was observed when light- and dark-roasted peanut flour slurries were preincubated at 90 °C or sonicated. The immunoreactivity of peanut allergens varied depending on the degre...Continue Reading
References
Feb 13, 1999·The Journal of Biological Chemistry·S J KoppelmanH H de Jongh
Nov 18, 2003·Allergy·S J KoppelmanS Piersma
Oct 28, 2004·Molecular Nutrition & Food Research·Roland E PomsElke Anklam
Apr 20, 2005·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S J KoppelmanE F Knol
Dec 24, 2005·The Biochemical Journal·Katrin LehmannPaul Rösch
Sep 9, 2011·PloS One·Yvonne M VissersKarine Adel-Patient
Nov 3, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·J RudolfS Baumgartner
Apr 11, 2012·Journal of Agricultural and Food Chemistry·Sefat KhudaKristina M Williams
Mar 12, 2013·Journal of Agricultural and Food Chemistry·Tong-Jen Fu, Nicole Maks
Nov 5, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Steve L TaylorGeert Houben
Aug 31, 2014·Food Chemistry·Sefat E KhudaKristina M Williams
Feb 5, 2015·Journal of Agricultural and Food Chemistry·Shyamali JayasenaStef J Koppelman
Oct 17, 2015·Food Chemistry·Lamia L'Hocine, Mélanie Pitre
Nov 26, 2015·Journal of Agricultural and Food Chemistry·Christine H ParkerLauren S Jackson
Feb 3, 2016·Food Chemistry·Adeseye O Lasekan, Balunkeswar Nayak
Oct 16, 2016·Journal of Food Science·Amma Amponsah, Balunkeswar Nayak
Dec 17, 2016·Food Chemistry·Nicole E WalczykThomas H Roberts
Dec 23, 2016·Preventive Medicine·Sameera A TalegawkarAlka M Kanaya
Apr 11, 2017·The American Journal of Geriatric Psychiatry : Official Journal of the American Association for Geriatric Psychiatry·Sandipan BhattacharjeeWilliam J Burke
Jul 16, 2017·The Journal of Allergy and Clinical Immunology·Stephanie FilepMartin D Chapman
Apr 1, 2007·Comprehensive Reviews in Food Science and Food Safety·Hsiao-Wei WenRichard A Durst