Extraction, isolation, and SDS-PAGE analysis of purified gastric mucin in a patient with Menetrier's disease

The American Journal of Gastroenterology
Anwar S MallR Hickman

Abstract

Menetrier's disease is a rare condition characterized by marked proliferation of gastric mucosa with variable mucus secretion and achlorhydria. Although crude mucus secretion and gastric aspirates have been evaluated in this disease for output of dry matter, hexosamine, fucose, protein content, and transforming growth factor alpha activity, we report for the first time the isolation, purification, and gel electrophoresis of mucin from crude mucus scrapings. The fragmentation pattern of mucin in Menetrier's disease demonstrated less large polymeric mucin than the control. There was also a band of approximately 55-65 kd M, on polyacrylamide gel electrophoresis similar to that found in gastric carcinoma or peptic ulcer, but absent in the control specimens.

References

Jan 1, 1978·Biochemical Society Transactions·M Mantle, A Allen
Dec 1, 1988·Annals of Surgery·T M SundtR A Malt
Nov 1, 1985·Digestive Diseases and Sciences·A Allen, N J Carroll
Apr 1, 1983·The Biochemical Journal·I CarlstedtL Wingerup
Jan 1, 1982·Advances in Experimental Medicine and Biology·A AllenJ P Pearson

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Citations

Jun 11, 2003·Journal of Gastroenterology and Hepatology·Anwar S MallDelawir Kahn
May 21, 2020·Macromolecular Bioscience·Carolin Alexandra RickertOliver Lieleg

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