PMID: 18727535Aug 30, 2008Paper

Extraction of aflatoxins B1 and G1 from maize by using aqueous sodium dodecyl sulfate

Journal of AOAC International
Chris M Maragos

Abstract

Aflatoxins are potent carcinogens produced by certain Aspergillus fungi. The aflatoxins were first discovered in the 1960s, and since then have been found to be distributed worldwide in a variety of commodities, foods, and feeds. Many of the early techniques for detecting aflatoxins involved extraction with halogenated solvents. With the increased availability and use of reversed-phase solid-phase extraction cartridges and the availability of immunoaffinity columns, aqueous mixtures of nonhalogenated solvents have been frequently used. To further reduce the need for solvents, we examined the effects of eliminating solvents during the extraction of maize, using aqueous mixtures of the detergent sodium dodecyl sulfate. After extraction and filtration, aflatoxins B1 (AFB1) and G1 (AFG1) were isolated by using commercially available immunoaffinity columns. The isolated AFB1 and AFG1 were derivatized with trifluoroacetic acid before separation by liquid chromatography with fluorescence detection. In spiked maize, the limits of detection were 0.5 and 1 ng/g for AFB1 and AFG1, respectively. Recoveries of AFB1 from maize spiked at 1-20 ng/g averaged 87.5% (range, 76.3-99.0%), with an average repeatability relative standard deviation (R...Continue Reading

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