Extraction of Bioactive Compounds Using Supercritical Carbon Dioxide

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Antonio MolinoDino Musmarra

Abstract

Microalgae Dunaliella salina contains useful molecules such as β-carotene and fatty acids (FAs), which are considered high value-added compounds. To extract these molecules, supercritical carbon dioxide was used at different operative conditions. The effects of mechanical pre-treatment (grinding speed at 0⁻600 rpm; pre-treatment time of 2.5⁻7.5 min) and operating parameters for extraction, such as biomass loading (2.45 and 7.53 g), pressure (100⁻550 bars), temperature (50⁻75 °C) and CO₂ flow rate (7.24 and 14.48 g/min) by varying the extraction times (30⁻110 min) were evaluated. Results showed that the maximum cumulative recovery (25.48%) of β-carotene was achieved at 400 bars and 65 °C with a CO₂ flow rate of 14.48 g/min, while the highest purity for stage (55.40%) was attained at 550 bars and 65 °C with a CO₂ flow rate of 14.48 g/min. The maximum recovery of FAs, equal to 8.47 mg/g, was achieved at 550 bars and 75 °C with a CO₂ flow rate of 14.48 g/min. Moreover, the lowest biomass loading (2.45 g) and the first extraction cycle (30 min) allowed the maximum extraction of β-carotene and FAs.

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Nov 6, 2018·International Journal of Environmental Research and Public Health·Antonio MolinoDino Musmarra

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Citations

Jul 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Laura ChronopoulouCleofe Palocci
Apr 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sanjeet MehariyaAntonio Molino
May 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Robert K HofstetterAndreas Link
Jan 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Brendan M Duggan
Jun 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mayya RazgonovaKirill Golokhvast

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