Extraction of Lipids from Liquid Biological Samples for High-Throughput Lipidomics.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Samuel FurseA. Koulman

Abstract

Extraction of the lipid fraction is a key part of acquiring lipidomics data. High-throughput lipidomics, the extraction of samples in 96w plates that are then run on 96 or 384w plates, has particular requirements that mean special development work is needed to fully optimise an extraction method. Several methods have been published as suitable for it. Here, we test those methods using four liquid matrices: milk, human serum, homogenised mouse liver and homogenised mouse heart. In order to determine the difference in performance of the methods as objectively as possible, we used the number of lipid variables identified, the total signal strength and the coefficient of variance to quantify the performance of the methods. This showed that extraction methods with an aqueous component were generally better than those without for these matrices. However, methods without an aqueous fraction in the extraction were efficient for milk samples. Furthermore, a mixture containing a chlorinated solvent (dichloromethane) appears to be better than an ethereal solvent (tert-butyl methyl ether) for extracting lipids. This study suggests that a 3:1:0.005 mixture of dichloromethane, methanol and triethylammonium chloride, with an aqueous wash, is ...Continue Reading

References

Oct 1, 1980·Journal of Biochemical and Biophysical Methods·M I Shoukry
Feb 19, 2008·Journal of Lipid Research·Vitali MatyashDominik Schwudke
Jul 31, 2010·Journal of Lipid Research·Oswald QuehenbergerEdward A Dennis
Sep 2, 2014·Metabolomics : Official Journal of the Metabolomic Society·Albert KoulmanDavid B Dunger
Jun 20, 2015·Metabolites·Zahir H AlshehryPeter J Meikle
Jul 28, 2015·Molecular Membrane Biology·Samuel FurseJ Antoinette Killian
Jan 28, 2018·Clinical and Translational Medicine·Todd A Lydic, Young-Hwa Goo
Jun 24, 2018·Progress in Lipid Research·Zhiqian LiuBen Cocks
Aug 29, 2018·Proceedings of the National Academy of Sciences of the United States of America·Adam J WatkinsKevin D Sinclair
Nov 23, 2018·Progress in Lipid Research·Adiilah Mamode CassimSébastien Mongrand
Mar 8, 2020·Analytical and Bioanalytical Chemistry·Samuel FurseAlbert Koulman

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Citations

May 17, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Milada VítováSamuel Furse
Jul 14, 2021·Analytical and Bioanalytical Chemistry·Samuel FurseAlbert Koulman

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