PMID: 708781Sep 21, 1978Paper

Extraction of mitochondrial proteins by volatile anesthetics

Biochimica Et Biophysica Acta
J J RichterS Kornguth

Abstract

Heavy beef heart mitochondria were exposed to controlled concentrations of several volatile anesthetics including halothane, enflurane and chloroform. These anesthetics caused a concentration-dependent release of protein from mitochondria with maximal release occurring at 17.5% halothane and less release at lower and higher concentrations. The proteins released into the supernatants were analyzed by electrophoresis on slab gels containing a 6--20% gradient of acrylamide. The anesthetics caused the release of several polypeptides from mitochondria into the incubation medium; the major polypeptides released had molecular weights of 78 000; 48 000; 47 000; 43 000; 32 000 and 22 000. Two of these were identified by enzyme analysis and by co-electrophoresis with crystalline enzymes as the subunits of aspartate aminotransferase (43 000 daltons; EC 2.6.1.1) and malate dehydrogenase (32 000 daltons; EC 1.1.1.37). Mitochondria exposed to saturated halothane vapors were similar ultrastructurally to controls except that the halothane mitochondria appeared uncoupled. Similar results were obtained with O2 or N2 as carrier gas.

Citations

Jan 1, 1988·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A A van IerselB J Blaauboer
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E A el-MaghrabiH Shuman
Apr 1, 1984·Neurochemical Research·S C ChengI A Minieka

Related Concepts

Anesthetic Effect
Bos indicus
Chloroform
Enlirane
Narcotan
Cell Surface Proteins
Mitochondria, Heart
Muscle Proteins

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