Extraction, purification and characterisation of exopolysaccharides produced by newly isolated lactic acid bacteria strains and the examination of their influence on resistant starch formation.

Food Chemistry
Krzysztof BuksaJakub Boreczek

Abstract

Exopolysaccharides (EPS) were produced by four newly isolated lactic acid bacteria strains, then further extracted, separated and characterised under standardised conditions. Using a sucrose carbon source, these LAB strains belonging to Weissella confusa/cibaria produced EPS with a dextran high molecular weight fraction. The obtained yields of EPS ranged from 3.2 g/L to 47.1 g/L and outstandingly high yields were obtained using Weissella confusa/cibaria 3MI3 isolated from spontaneous spelt sourdough. After purification the influence of EPS-dextran of molar mass 3,244,000 g/mol on resistant starch formation in wheat starch pastes and pasted samples after temperature-cycled storage was examined. Size exclusion chromatography with post-column derivatisation revealed that a 1.5% share of EPS dextran limited formation of high molar mass resistant starch in starch pastes during storage. This work provides new insight on hindering resistant starch formation by using EPS, which could be efficiently produced in sourdough, thus improving the properties of sourdough bread.

References

Mar 31, 2005·Advances in Food and Nutrition Research·Charmaine I Clarke, Elke K Arendt
Sep 30, 2006·Food Microbiology·G LacazeS Cappelle
Sep 15, 2009·Food Microbiology·Kati KatinaArja Laitila
Mar 13, 2014·International Journal of Biological Macromolecules·Long ChenGuilan Zhu
Mar 19, 2014·Applied and Environmental Microbiology·Raffaella Di CagnoMaria Calasso
Apr 24, 2015·Applied Biochemistry and Biotechnology·Dilna Sasidharan VasanthakumariKesavan Madhavan Nampoothiri
Aug 26, 2015·Food Chemistry·Krzysztof BuksaRafał Ziobro
Jun 21, 2016·International Journal of Food Microbiology·Xiao Yan ChenMichael G Gänzle
Aug 24, 2017·FEMS Microbiology Reviews·Ahmad A ZeidanAna Rute Neves
Oct 18, 2019·Journal of Nutritional Science and Vitaminology·Yusuke YamaguchiHitomi Kumagai

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