Extraction, Separation, and Identification of Phenolic Compounds in Virgin Olive Oil by HPLC-DAD and HPLC-MS

Antioxidants
Maria Tasioula-Margari, Eleftheria Tsabolatidou

Abstract

The aim of this study was to evaluate the recovery of individual phenolic compounds extracted from virgin olive oil (VOO), from different Greek olive varieties. Sufficient recoveries (90%) of all individual phenolic compounds were obtained using methanol as an extraction solvent, acetonitrile for residue solubilization, and two washing steps with hexane. Moreover, in order to elucidate structural characteristics of phenolic compounds in VOO, high performance liquid chromatography with a diode array detector (HPLC-DAD) at 280 and 340 nm and HPLC coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS) in the negative-ion mode were performed. The most abundant phenolic compounds were oleuropein derivatives with m/z 319 and 377 and ligstroside derivatives with m/z 303, 361. Lignans, such as 1-acetoxypinoresinol and pinoresinol were also present in substantial quantities in the phenolic fraction. However, pinoresinol was co-eluted with dialdehydic form of ligstroside aglycone (DAFLA) and it was not possible to be quantified separately. The phenolic extracts, obtained from different VOO samples, yielded similar HPLC profiles. Differences, however, were observed in the last part of the chromatogram, corresponding to isomers...Continue Reading

References

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Citations

Feb 8, 2016·Plant Foods for Human Nutrition·Maria Lisa ClodoveoCarlo Franchini
Feb 14, 2017·Journal of Agricultural and Food Chemistry·Gianfranco BazzuPier Andrea Serra
Jul 18, 2018·Preparative Biochemistry & Biotechnology·Ekant TamboliAbha Mishra
Oct 10, 2019·Nutritional Neuroscience·Morgane Lambert de MalezieuCharles Ramassamy
Sep 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Delgadillo Puga ClaudiaPedraza-Chaverri José
Aug 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Antonio FranciosoLuciana Mosca
Sep 9, 2017·Journal of Cellular Physiology·Tiziana SquillaroMariarosa A B Melone
Dec 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chiara FanaliLaura de Gara
Jan 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cecilia Castro-LópezVíctor E Aguirre-Arzola
Apr 27, 2018·Journal of Agricultural and Food Chemistry·Eugenia PapadakiFani Th Mantzouridou
Oct 22, 2021·Frontiers in Nutrition·Daniela GabbiaSara De Martin

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Methods Mentioned

BETA
Nuclear Magnetic Resonance

Software Mentioned

SPSS

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