Extractionless high-performance liquid chromatographic method for the simultaneous determination of piroxicam and 5'-hydroxypiroxicam in human plasma and urine

Journal of Chromatography. B, Biomedical Applications
A AvgerinosS Malamataris

Abstract

A simple and rapid (extractionless) high-performance liquid chromatographic method with UV detection, at 330 nm, was developed for the simultaneous determination of piroxicam and its major metabolite, 5'-hydroxypiroxicam, in human plasma and urine. Acidified plasma and alkali-treated urine samples are used and naproxen is added as internal standard. The separation is performed at 40 degrees C on a C18 Spherisorb column with acetonitrile--0.1 M sodium acetate (33:67, v/v, pH 3.3) as mobile phase. The retention time is 2.2 min for 5'-hydroxypiroxicam, 2.6 min for piroxicam and 3.2 min for naproxen. The detection limit is 0.05 micrograms/ml using a 100-microliters loop.

References

Sep 9, 1988·Journal of Chromatography·F D Boudinot, S S Ibrahim
Jul 11, 1980·Journal of Chromatography·T M TwomeyD C Hobbs

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Citations

Jun 25, 1998·Journal of Pharmaceutical and Biomedical Analysis·P C DamianiA C Olivieri
Apr 18, 2000·Journal of Pharmaceutical and Biomedical Analysis·S P Puthli, P R Vavia
Feb 19, 2002·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·María Isabel Pascual-RegueraMaría Soledad Castro Ruiz
May 30, 2007·Clinical Drug Investigation·Miriam Del Carmen Carrasco-PortugalFrancisco J Flores-Murrieta
Mar 4, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hamid AbdollahiAbdolhossein Naseri
May 26, 2016·Brazilian Oral Research·Adriana Maria CalvoCarlos Ferreira Santos
Jun 10, 2014·Animal : an International Journal of Animal Bioscience·B AgnvallP Jensen
Jul 20, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·A BakkaliF Vicente

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