Extraembryonic but not embryonic SUMO-specific protease 2 is required for heart development

Scientific Reports
Eri O MaruyamaWei Hsu

Abstract

SUMO-specific protease 2 (SENP2) activities to remove SUMO from its substrates is essential for development of trophoblast stem cells, niches and lineages. Global deletion of SENP2 leads to midgestation lethality, and causes severe defects in the placenta which is accompanied by embryonic brain and heart abnormalities. Because of the placental deficiencies, the role of SENP2 in development of the embryonic tissues has not been properly determined. The brain and heart abnormalities may be secondary to placental insufficiency. Here we have created a new mouse strain permitting conditional inactivation of SENP2. Mice homozygous for germline deletion of the conditional allele exhibit trophoblast defects and embryonic abnormalities resembling the global SENP2 knockout. However, tissue-specific disruptions of SENP2 demonstrate its dispensable role in embryogenesis. Placental expression of SENP2 is necessary and sufficient for embryonic heart and brain development. Using a protease deficient model, we further demonstrate the requirement of SENP2-dependent SUMO modification in development of all major trophoblast lineages. SENP2 regulates sumoylation of Mdm2 which controls p53 activities critical for G-S transition of mitotic division ...Continue Reading

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Citations

May 19, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Takamitsu MaruyamaWei Hsu
Oct 19, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H-M Ivy YuWei Hsu
Apr 21, 2020·Biochemical Society Transactions·Ana TalamilloRosa Barrio
Mar 15, 2018·Nature·Vicente Perez-GarciaMyriam Hemberger
Apr 28, 2017·BMC Developmental Biology·Dorota SzumskaShoumo Bhattacharya
Jul 13, 2018·Frontiers in Physiology·Emily J CammAmanda N Sferruzzi-Perri
Jul 3, 2018·Frontiers in Physiology·Cheryl L Maslen

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Methods Mentioned

BETA
sumoylation
PCR
gene trap
genotyping
electrophoresis

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