Extrahypothalamic brain luteinizing hormone: characterization by radioimmunoassay, chromatography, radioligand assay and bioassay

N V EmanueleA M Lawrence


We have recently reported that luteinizing hormone (LH) is present in the hypothalamus of rats. It has chromatographic and biologic characteristics similar to pituitary LH. In this report we focus on extrahypothalamic LH that is widely distributed in the rodent central nervous system. This material has a chromatographic profile similar to that of pituitary LH. Serial dilution of this material is parallel with dilutions of rat pituitary LH in the immunoassay. Brain extracts are active in the testis LH radioligand receptor assay and in the rat interstitial cell testosterone secretion bioassay. Prior incubation of extract with LH antibody significantly attenuated both of these activities. Thus, extrahypothalamic LH has immunologic, chromatographic, and biologic characteristics similar to hypothalamic and pituitary LH.


Jan 15, 1993·Experientia·S PajovićJ V Martinović
Dec 23, 1985·Brain Research·S HojvatA M Lawrence
Mar 31, 1987·Brain Research·N V EmanueleA M Lawrence
Jul 15, 2015·Hormones and Behavior·Jeffrey A BlairGemma Casadesus
Apr 1, 1992·Journal of Pineal Research·H P NotebornC A Salemink
Jan 1, 1990·Endocrine Research·M R KelleyN V Emanuele
Oct 16, 2018·Frontiers in Endocrinology·Sabina BhattaGemma Casadesus

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Brain Chemistry
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