Extremely sensitive sandwich assay of kanamycin using surface-enhanced Raman scattering of 2-mercaptobenzothiazole labeled gold@silver nanoparticles

Analytica Chimica Acta
Adem ZenginTuncer Caykara

Abstract

Herein, we report the development of extremely sensitive sandwich assay of kanamycin using a combination of anti-kanamycin functionalized hybrid magnetic (Fe3O4) nanoparticles (MNPs) and 2-mercaptobenzothiazole labeled Au-core@Ag-shell nanoparticles as the recognition and surface-enhanced Raman scattering (SERS) substrate, respectively. The hybrid MNPs were first prepared via surface-mediated RAFT polymerization of N-acryloyl-L-glutamic acid in the presence of 2-(butylsulfanylcarbonylthiolsulfanyl) propionic acid-modified MNPs as a RAFT agent and then biofunctionalized with anti-kanamycin, which are both specific for kanamycin and can be collected via a simple magnet. After separating kanamycin from the sample matrix, they were sandwiched with the SERS substrate. According to our experimental results, the limit of detection (LOD) was determined to be 2pg mL(-1), this value being about 3-7 times more than sensitive than the LOD of previously reported results, which can be explained by the higher SERS activity of silver coated gold nanoparticles. The analysis time took less than 10min, including washing and optical detection steps. Furthermore, the sandwich assay was evaluated for investigating the kanamycin specificity on neomyc...Continue Reading

Citations

Dec 4, 2015·The Analyst·Kristy S McKeatingJean-Francois Masson
Sep 9, 2015·Analytica Chimica Acta·Faten FaroukWilfried M A Niessen
Jul 15, 2016·The Analyst·Kristy S McKeatingJean-Francois Masson
Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal
Nov 30, 2018·Nanomaterials·Pengfei JiangLibo Nie

Related Concepts

Altax
Bos indicus
Chemistry Techniques, Analytical
Gold
Kantrex
Silver
Raman Optical Activity Spectroscopy
Benzothiazoles
Magnetite
Metal Nanoparticles

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