Extrusion mechanisms of intracellular Ca2+ in human aortic endothelial cells

European Journal of Pharmacology
Y GotoT Iijima

Abstract

Although participation of the plasma membrane Ca2+ pump in Ca2+ efflux has been generally accepted, the existence of Na(2+)-Ca2+ exchange in endothelial cells is still controversial. In the present experiments, the role of Na(+)-Ca2+ exchange and Ca(2+)-ATPase were examined in cultured human aortic endothelial cells loaded with fura. In Ca(2+)-free solution, the declining phase of [Ca2+]i in response to histamine was clearly slowed with low Na+ solution or Ni2+. Vanadate also slightly slowed the declining phase. The declining phase was much more clearly slowed with La3+. The response to thapsigargin, a specific endoplasmic reticulum Ca2+ ATPase inhibitor, was much more clearly prolonged by those interventions. These results strongly imply the presence of Na(2+)-Ca2+ exchange and Ca2+ ATPase in the plasma membrane, and suggest that not only Ca2+ uptake into the internal stores but also Na(+)-Ca2+ exchange and plasma membrane Ca2+ ATPase have a physiological role in reducing [Ca2+]i elevated by receptor agonists and endoplasmic reticulum Ca(2+)-ATPase inhibitors in cultured human aortic endothelial cells.

References

Jul 27, 1978·Nature·R DiPolo
Aug 15, 1991·Archives of Biochemistry and Biophysics·K K WangT H Kuo
Jan 1, 1991·Annals of the New York Academy of Sciences·B A HansenM E O'Donnell
Jan 1, 1991·The Journal of Physiology·S O SageM B Cannell
Jan 1, 1991·Physiological Reviews·E Carafoli
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
May 9, 1989·Biochimica Et Biophysica Acta·G J KaczorowskiM L Garcia
Apr 1, 1982·The Journal of Physiology·W H Barry, T W Smith
Feb 21, 1980·Nature·P Caroni, E Carafoli
Mar 1, 1995·Circulation Research·L Li, C van Breemen
Jun 1, 1994·Pflügers Archiv : European journal of physiology·E C Toescu, O H Petersen
Feb 15, 1994·European Journal of Pharmacology·E Hosoki, T Iijima

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Citations

Aug 21, 2012·World Journal of Biological Chemistry·Francesco MocciaFranco Tanzi
Feb 9, 2011·Progress in Biophysics and Molecular Biology·Marco SciannaLuigi Preziosi
Feb 5, 2008·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Ganesan MuraliChinnakkannu Panneerselvam
Jun 12, 2004·Clinical and Experimental Pharmacology & Physiology·Xian Hui DongHakuo Takahashi
May 21, 2013·Journal of Theoretical Biology·M SciannaL Preziosi
Apr 16, 2004·Journal of Cardiovascular Pharmacology·Alexander SchusterJean-Jacques Meister
Dec 9, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mauricio A LilloXavier F Figueroa
Feb 15, 2008·Journal of Biochemical and Molecular Toxicology·Carlo A PalmeriniGiuseppe Arienti

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