Extrusion of Ca2+ from mouse motor terminal mitochondria via a Na+-Ca2+ exchanger increases post-tetanic evoked release.

The Journal of Physiology
Luis García-ChacónEllen F Barrett

Abstract

Mitochondria sequester much of the Ca2+ that enters motor nerve terminals during repetitive stimulation at frequencies exceeding 10-20 Hz. We studied the post-stimulation extrusion of Ca2+ from mitochondria by measuring changes in matrix [Ca2+] with fluorescent indicators loaded into motor terminal mitochondria in the mouse levator auris longus muscle. Trains of action potentials at 50 Hz produced a rapid increase in mitochondrial [Ca2+] followed by a plateau, which was usually maintained after the end of the stimulus train and then slowly decayed back to baseline. Increasing the Ca2+ load delivered to the terminal by increasing the number of stimuli (from 500 to 2000) or the stimulation frequency (from 50 to 100 Hz), by increasing bath [Ca2+], or by prolonging the action potential with 3,4-diaminopyridine (100 microM) prolonged the post-stimulation decay of mitochondrial [Ca2+] without increasing the amplitude of the plateau during stimulation. Inhibiting the opening of the mitochondrial permeability transition pore with cyclosporin A (5 microM) had no significant effect on the decay of mitochondrial [Ca2+]. Inhibition of the mitochondrial Na+-Ca2+ exchanger with CGP-37157 (50 microM) dramatically prolonged the post-stimulatio...Continue Reading

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Citations

Feb 5, 2010·Apoptosis : an International Journal on Programmed Cell Death·Hansruedi Büeler
Nov 18, 2011·Journal of Bioenergetics and Biomembranes·Ellen F BarrettGavriel David
Jul 24, 2012·The Journal of Biological Chemistry·Raz PaltyIsrael Sekler
Oct 2, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Man-Su Kim, Yuriy M Usachev
Feb 5, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amit K ChouhanGregory T Macleod
May 16, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yuliya V MedvedevaYuriy M Usachev
Jan 29, 2009·Proceedings of the National Academy of Sciences of the United States of America·Khanh T NguyenGavriel David
Mar 21, 2012·Cell Calcium·Raz Palty, Israel Sekler
Oct 4, 2016·Frontiers in Synaptic Neuroscience·Seok-Kyu KwonFranck Polleux
Oct 22, 2008·Methods : a Companion to Methods in Enzymology·R Madelaine ParedesJames D Lechleiter
Jan 1, 2014·Genesis : the Journal of Genetics and Development·Vishal Sharma, Damien M O'Halloran
Mar 6, 2010·Journal of Neurochemistry·Ricardo de Freitas LimaChristopher Kushmerick
Nov 10, 2006·American Journal of Physiology. Cell Physiology·Oliver Kann, Richard Kovács
Sep 15, 2015·The Journal of Physiology·Zhongsheng Zhang, Gavriel David
Dec 14, 2006·The Journal of Biological Chemistry·Haider F Altimimi, Paul P M Schnetkamp
Mar 12, 2021·Human Molecular Genetics·Mario Lopez-ManzanedaLucia Tabares

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