Exudative neutrophils show impaired pH regulation compared with circulating neutrophils

Archives of Surgery
D J HackamO D Rotstein

Abstract

To investigate the effects of neutrophil (PMN) transmigration into inflammatory sites on cytosolic pH (pHi) regulation. Exudative PMNs were obtained by bronchoalveolar lavage from rats sustaining endotoxin-induced lung injury. Circulating PMNs were purified with density-gradient centrifugation. Cytosolic pH was measured with single-cell fluorescence imaging using the pH-sensitive dye biscarboxyethyl-carboxyfluorescein. Exudative PMNs showed impaired pHi recovery from an induced acid load compared with circulating PMNs. Under conditions of extracellular acidosis, exudative PMNs showed impaired pHi homeostasis and produced decreased superoxide compared with circulating cells. Inhibition of the sodium-proton exchanger attenuated the differences in pHi recovery, suggesting a mechanism underlying the pHi regulatory dysfunction. All cells had comparable adenosine triphosphate levels and superoxide production at physiologic extracellular pH. Impaired pHi regulation of exudative cells may mediate cellular dysfunction and impaired resolution of infection at inflammatory sites.

Citations

Feb 8, 2005·Critical Care : the Official Journal of the Critical Care Forum·Donall O'CroininJohn G Laffey
Nov 2, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Liu YangXin Guan
Jan 31, 2009·Journal of Burn Care & Research : Official Publication of the American Burn Association·David J Dries
Jul 13, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·R J CoakleyN G McElvaney

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