PMID: 2103308Jan 1, 1990Paper

Eye muscle membrane reactive antibodies are not detected in the serum or immunoglobulin fraction of patients with thyroid-associated ophthalmopathy using an ELISA and crude membranes

Autoimmunity
M KapustaJ R Wall

Abstract

We tested sera and purified immunoglobulin (Ig) fractions from patients with autoimmune thyroid disorders (AITD), with and without ophthalmopathy, and normal subjects, for the presence of antibodies reactive with eye muscle membrane antigens in an optimized enzyme-linked immunosorbent assay (ELISA). We found no correlation between ELISA results and the presence or severity of ophthalmopathy in patients with AITD for either serum or Ig, and there were no significant differences between the mean values (+/- SE) for the three groups (AITD with ophthalmopathy, AITD without ophthalmopathy and normals) for either serum or Ig. In contrast Ig from 8 of 19 (45%) patients with thyroid-associated ophthalmopathy reacted with a 64 kDa eye muscle membrane antigen in SDS-polyacrylamide gel electrophoresis and Western blotting, while tests were positive in only one of the 8 patients with AITD without eye disease and in none of the 8 normal subjects. The presence of antibodies to a 64 kDa antigen in immunoblotting did not correlate with the levels of antibodies measured in ELISA. We conclude that the ELISA, incorporating a crude membrane fractions as antigen, is not useful as a clinical test for eye muscle autoantibodies.

References

Jul 1, 1988·Journal of Endocrinological Investigation·K BanovacS F Byrne
May 1, 1988·The Journal of Clinical Endocrinology and Metabolism·M SalviJ R Wall
Oct 1, 1986·The Journal of Clinical Endocrinology and Metabolism·M KadlubowskiA C Rowland

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Citations

Apr 16, 2002·Thyroid : Official Journal of the American Thyroid Association·Matthias KasparJack Ronald Wall
Dec 16, 1998·Thyroid : Official Journal of the American Thyroid Association·B JoffeJ R Wall
Feb 1, 1994·Journal of Endocrinological Investigation·J R WallN Bernard

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