PMID: 8947552Dec 1, 1996Paper

F-actin bundles in Drosophila bristles are assembled from modules composed of short filaments

The Journal of Cell Biology
L G TilneyG M Guild

Abstract

The actin bundles in Drosophila bristles run the length of the bristle cell and are accordingly 65 microns (microchaetes) or 400 microns (macrochaetes) in length, depending on the bristle type. Shortly after completion of bristle elongation in pupae, the actin bundles break down as the bristle surface becomes chitinized. The bundles break down in a bizarre way; it is as if each bundle is sawed transversely into pieces that average 3 microns in length. Disassembly of the actin filaments proceeds at the "sawed" surfaces. In all cases, the cuts in adjacent bundles appear in transverse register. From these images, we suspected that each actin bundle is made up of a series of shorter bundles or modules that are attached end-to-end. With fluorescent phalloidin staining and serial thin sections, we show that the modular design is present in nondegenerating bundles. Decoration of the actin filaments in adjacent bundles in the same bristle with subfragment 1 of myosin reveals that the actin filaments in every module have the same polarity. To study how modules form developmentally, we sectioned newly formed and elongating bristles. At the bristle tip are numerous tiny clusters of 6-10 filaments. These clusters become connected together ...Continue Reading

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Citations

Apr 12, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Nina KureishyJosephine Clare Adams
Mar 9, 2004·Journal of Molecular Biology·Jennifer H ShinPaul Matsudaira
May 16, 2003·Tissue & Cell·Ioannis P NezisLukas H Margaritis
Feb 19, 2000·Current Opinion in Cell Biology·J R Bartles
Nov 12, 2013·Nature Cell Biology·Ruei-Jiun HungJonathan R Terman
Jun 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Inna A BelyantsevaJames M Ervasti
Aug 22, 2001·Molecular Biology of the Cell·M SteketeeK W Tosney
Sep 17, 2004·Molecular Biology of the Cell·Lewis G TilneyDavid Derosier
May 27, 2005·Molecular Biology of the Cell·Gregory M GuildLewis G Tilney
Jan 1, 1997·Annual Review of Cell and Developmental Biology·D N Robinson, L Cooley
Jun 27, 2013·Cell Structure and Function·Akihito KawamoriMasamitsu Yamaguchi
Dec 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Lewis G Tilney, David J DeRosier
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·M EddisonJ Lewis
Sep 17, 2003·The Journal of Cell Biology·Gregory M GuildLewis G Tilney
Feb 21, 2002·The Journal of Cell Biology·Andrew M Hudson, Lynn Cooley
Jan 12, 2000·The Journal of Cell Biology·L G TilneyG M Guild
Dec 28, 2011·Hearing Research·Meghan C DrummondThomas B Friedman
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Mar 29, 2007·The Journal of Cell Biology·Sangil Lee, Lynn Cooley

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