PMID: 8967337Apr 1, 1996Paper

F-actin disorganization in apoptotic cell death of cultured rat renal proximal tubular cells

The American Journal of Physiology
B van de WaterJ F Nagelkerke

Abstract

The mechanism of nephrotoxin-induced apoptosis was studied in rat renal proximal tubular cells (PTC) exposed to the nephrotoxin S-(1,2-dichlorovinyl)-L-cysteine (DCVC). After a 6-h incubation, DCVC caused a condensation of heterochromatin and a fragmentation of the nucleus in 84 and 16% of the cells, respectively, which is indicative of apoptosis. This was confirmed biochemically by agarose gel electrophoresis demonstrating the formation of DNA fragments with multiples of 200 bp. The antioxidant N,N'-diphenyl-p-phenylenediamine prevented neither the fragmentation of the nucleus nor the formation of DNA fragments, but it did prevent lactate dehydrogenase release and bleb formation by DCVC. Apoptosis induced by DCVC was closely associated with F-actin disorganization: every cell with a fragmented nucleus displayed completely disorganized F-actin, while cells with a normal nucleus still possessed at least some intact F-actin also induced apoptosis in PTC. Similarly, dithiothreitol, which damages F-actin in PTC, caused apoptosis of PTC. These data suggest a causal relationship between F-actin disorganization and apoptosis of PTC.

Citations

Jan 11, 2002·Molecular and Cellular Biology·Catherine PaulAndré-Patrick Arrigo
Jul 26, 2019·International Journal of Molecular Sciences·Giovanna PrianteFranca Anglani
Aug 12, 2005·World Journal of Gastroenterology : WJG·Jian-Min SuXi-Liang Zha
Sep 18, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Saleem AladailehDavid A Raftos
Jun 15, 2011·Fish & Shellfish Immunology·Rhiannon P Kuchel, David A Raftos

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis