F-actin network formation in tethers and in pseudopods stimulated by chemoattractant

Cell Motility and the Cytoskeleton
Doncho V ZhelevR M Hochmuth

Abstract

Micropipets are used either to deliver a given concentration of the chemoattractant N-formyl-methionyl-leucyl-phenylalanine (fMLP) to a local region of a human neutrophil or to create a membrane tether. Pseudopods, which have a cylindrical shape and grow at a constant rate, are formed in either case. After reaching a maximum extension, they retract, even in the presence of chemoattractant. As a pseudopod grows, cell granules begin to penetrate the pseudopod region to a "boundary" that defines a distance to the pseudopod's leading edge that is almost constant. The exclusion of granules from this domain indicates that it is filled with a dense network. The formation of this network involves the plasma membrane because pseudopod growth ceases when a membrane tether is pulled away from the leading edge. The rate of pseudopod growth depends on fMLP concentration just as the number of occupied N-formyl peptide receptors depends on this concentration. The experimental data are explained by assuming that F-actin network is formed next to the plasma membrane. The newly formed network displaces the membrane and the dominant process in the network region then becomes F-actin depolymerization. The rate of pseudopod growth is determined by ...Continue Reading

References

Jan 1, 1992·Cell Motility and the Cytoskeleton·J Condeelis
Aug 2, 1990·Nature·T A Springer
Jul 11, 1991·Nature·J A Theriot, T J Mitchison
Jan 1, 1988·Cell Motility and the Cytoskeleton·J R SimonD L Taylor
Jan 1, 1988·Annual Review of Cell Biology·P N Devreotes, S H Zigmond
Dec 1, 1988·Biophysical Journal·C Zhu, R Skalak
Jan 1, 1987·Physiological Reviews·G M OmannL A Sklar
Aug 1, 1994·Biophysical Journal·D V ZhelevR M Hochmuth
Jan 1, 1994·Cell Motility and the Cytoskeleton·S Felder, Z Kam
Jan 1, 1993·Annual Review of Cell Biology·J Condeelis
Jan 1, 1993·Cell Motility and the Cytoskeleton·S H Zigmond
Jan 1, 1993·The Journal of Cell Biology·H P KaoA S Verkman
May 21, 1993·Science·T P Stossel
Apr 1, 1993·Biophysical Journal·E Evans

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Citations

Apr 17, 2013·Gastroenterology Research and Practice·Khanh Vinh Quốc L Ng, Lan Thi Hoàng Nguyễn
Jul 13, 2006·Annual Review of Biomedical Engineering·Geert W Schmid-Schönbein
Jan 30, 2008·Current Protocols in Cell Biology·S H ZigmondJ E Segall
May 28, 2004·American Journal of Physiology. Cell Physiology·M YanaiH Higuchi
Dec 22, 1999·The Journal of Biological Chemistry·B HaribabuR Snyderman
Jan 5, 2000·Journal of Immunological Methods·M Torres, T D Coates
Jul 9, 2004·Biophysical Journal·Doncho V ZhelevDavid Chodniewicz
Apr 30, 2003·Biophysical Journal·Marc HerantMicah Dembo

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