F-box protein CFK1 interacts with and degrades de novo DNA methyltransferase in Arabidopsis.

The New Phytologist
Jiani ChenXuehua Zhong

Abstract

DNA methylation plays crucial roles in cellular development and stress responses through gene regulation and genome stability control. Precise regulation of DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2), the de novo Arabidopsis DNA methyltransferase, is crucial to maintain DNA methylation homeostasis to ensure genome integrity. Compared with the extensive studies on DRM2 targeting mechanisms, little information is known regarding the quality control of DRM2 itself. Here, we conducted yeast two-hybrid screen assay and identified an E3 ligase, COP9 INTERACTING F-BOX KELCH 1 (CFK1), as a novel DRM2-interacting partner and targets DRM2 for degradation via the ubiquitin-26S proteasome pathway in Arabidopsis thaliana. We also performed whole genome bisulfite sequencing (BS-seq) to determine the biological significance of CFK1-mediated DRM2 degradation. Loss-of-function CFK1 leads to increased DRM2 protein abundance and overexpression of CFK1 showed reduced DRM2 protein levels. Consistently, CFK1 overexpression induces genome-wide CHH hypomethylation and transcriptional de-repression at specific DRM2 target loci. This study uncovered a distinct mechanism regulating de novo DNA methyltransferase by CFK1 to control DNA methylation level.

References

Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Feb 24, 2001·Genome Biology·E T Kipreos, M Pagano
Mar 27, 2001·Nature Reviews. Genetics·K D Robertson, A P Wolffe
Jun 19, 2001·Medicinal Research Reviews·J MyungC M Crews
Apr 19, 2002·Nature·Ning ZhengNikola P Pavletich
Jul 18, 2002·Current Biology : CB·Xiaofeng Cao, Steven E Jacobsen
Sep 7, 2002·The Plant Cell·William M GrayMark Estelle
Sep 4, 2003·Genome Research·Li LiDavid S Roos
Dec 19, 2003·Current Biology : CB·Xiaofeng CaoSteven E Jacobsen
Dec 23, 2003·The Plant Cell·Fuquan LiuDaoxin Xie
Mar 17, 2004·PLoS Biology·Zhixin XieJames C Carrington
Jul 12, 2005·Nature Structural & Molecular Biology·Shahri RaasiCecile M Pickart
Sep 12, 2006·The Plant Journal : for Cell and Molecular Biology·Radhika DesikanSteven J Neill
Sep 21, 2006·Nature Reviews. Genetics·Robert J KloseYi Zhang
May 1, 2007·Current Opinion in Cell Biology·Michael Weber, Dirk Schübeler
Aug 4, 2007·Science·Magnolia BostickSteven E Jacobsen
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rebecca A MosherDavid C Baulcombe
Dec 4, 2008·American Journal of Physiology. Endocrinology and Metabolism·Xu Tan, Ning Zheng
May 27, 2009·Cellular and Molecular Life Sciences : CMLS·Vivian Su, Alan F Lau
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Jul 29, 2009·BMC Bioinformatics·Yuanxin Xi, Wei Li
Dec 2, 2009·The Journal of Biological Chemistry·Yuanbiao ZhaoQun He
Feb 10, 2010·Nature Reviews. Genetics·Julie A Law, Steven E Jacobsen
Apr 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Suhua FengSteven E Jacobsen
Dec 15, 2010·Nature Structural & Molecular Biology·Pierre-Olivier EstèveSriharsa Pradhan
Mar 5, 2011·Annual Review of Plant Biology·Zhihua Hua, Richard D Vierstra
May 14, 2011·Biochemical and Biophysical Research Communications·Geneviève Lavoie, Yves St-Pierre
Jun 11, 2011·EMBO Reports·Hélène DenisFrançois Fuks
Mar 15, 2012·Seminars in Cell & Developmental Biology·Karin Flick, Peter Kaiser
Jun 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Robert H DowenJoseph R Ecker

❮ Previous
Next ❯

Citations

Apr 27, 2021·Frontiers in Plant Science·Jamie N OrrIsabelle Colas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.