F-Type ATP Synthase Assembly Factors Atp11 and Atp12 in Arabidopsis

Frontiers in Plant Science
Zhikun DuanLianwei Peng

Abstract

Atp11p and Atp12p are members of two chaperone families essential for assembly of the mitochondrial ATP synthase in Saccharomyces cerevisiae and Homo sapiens. However, the role of their homologs in higher plants is unclear with regard to the assembly of both chloroplast ATP synthase (cpATPase) and mitochondrial ATP synthase (mtATPase). Here, we show that loss of either Atp11 or Atp12 is lethal in Arabidopsis. While Atp12 is only localized in mitochondria, Atp11 is present both in chloroplasts and mitochondria. Yeast two-hybrid analyses showed that, as their homologs in yeast, Atp11 specifically interacts with the β subunit of the mtATPase and cpATPase, and Atp12 interacts with the α subunit of the mtATPase, implying that Atp11 and Atp12 fulfill a conserved task during assembly of ATP synthase. However, the binding sites for Atp11 in the β subunit of mtATPase and cpATPase are slightly different, suggesting that the mechanisms of action may have evolved in different ways. Although Atp11 interacts with cpATPase β subunit as the two assembly factors BFA3 and BFA1, they bind to different sites of the β subunit. These results indicate that Atp11 is involved in the assembly of both cpATPase and mtATPase but Atp12 is specifically requi...Continue Reading

References

Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S H Ackerman, A Tzagoloff
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Feb 22, 2000·The Journal of Biological Chemistry·Z G Wang, S H Ackerman
Oct 18, 2000·The Journal of Biological Chemistry·G Groth, E Pohl
Jun 19, 2001·The Journal of Biological Chemistry·Z G WangS H Ackerman
Sep 5, 2001·Nature Reviews. Molecular Cell Biology·M YoshidaT Hisabori
Sep 11, 2002·Biochimica Et Biophysica Acta·Sharon H Ackerman
Dec 6, 2003·Science·Katherine W Osteryoung, Jodi Nunnari
Jan 22, 2004·Nature Reviews. Genetics·Jeremy N TimmisWilliam Martin
Mar 25, 2005·Proteins·Andrea PíckováJosef Houstek
Sep 17, 2005·Progress in Nucleic Acid Research and Molecular Biology·Sharon H Ackerman, Alexander Tzagoloff
Mar 5, 2008·Methods in Molecular Biology·Lee J SweetloveChristopher J Leaver
Jun 3, 2008·Archives of Biochemistry and Biophysics·Robert K NakamotoMarwan K Al-Shawi
Apr 23, 2009·The Journal of Biological Chemistry·Anthony LudlamSharon H Ackerman
Jun 6, 2009·Annual Review of Biochemistry·Christoph von BallmoosPeter Dimroth
Jan 27, 2011·The EMBO Journal·Malgorzata RakAlexander Tzagoloff
Feb 11, 2015·Biochimica Et Biophysica Acta·Thilo Rühle, Dario Leister
Mar 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Juan MaoLixin Zhang
Apr 4, 2015·Annual Review of Biochemistry·Wolfgang Junge, Nathan Nelson
May 12, 2018·Science·Alexander HahnWerner Kühlbrandt
Jul 27, 2018·The Plant Cell·Gregory Bertoni
Mar 23, 2019·Annual Review of Biochemistry·Werner Kühlbrandt
Apr 30, 2019·Frontiers in Plant Science·Lin ZhangLianwei Peng
Apr 7, 2020·Journal of Integrative Plant Biology·Xuemei WangCongming Lu
Jun 12, 2020·BMC Plant Biology·Xiaohui QinChun-Peng Song

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Methods Mentioned

BETA
PCR
light microscopy
transgenic
Immunoprecipitation
two-hybrid
two hybrid
Protein Assay

Software Mentioned

TargetP
ChloroP analysis
TMHMM
ClustalW2
ChloroP

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