F3, a novel active fraction of Valeriana jatamansi Jones induces cell death via DNA damage in human breast cancer cells

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Zhihui ZhuHuajun Zhao

Abstract

F3 is a novel fraction, for the first time isolated from Valeriana jatamansi Jones, which is a traditional Chinese folk medicine. Its anti-cancer potential and the underlying molecular mechanisms have not been well elucidated. This study aims to investigate the anti-cancer effects of F3 on human breast cancer cell lines and its underlying mechanisms. MTT assay was first performed to detect the effect of F3 on cell viability in human breast cancer cell lines and human mammary epithelial MCF-10A cells. Cell apoptosis, mitochondrial membrane potential and ROS level were detected by flow cytometry. Comet and immunofluorescence assays were utilized to assess DNA damage and expression of γ-H2AX. Autophagy were observed by AO staining and fluorescence microscopy. The expression of relative proteins was detected by western blotting. The xenograft model in nude mice was used to elucidate the effect of F3 on tumor growth and DNA damage in vivo. F3 could significantly inhibit the growth of breast cancer cells in concentration-dependent manner by inducing apoptosis and has no obvious inhibitory effect of the growth on MCF-10A cells. Mechanistic studies demonstrated that F3-induced apoptosis was mediated by DNA damage as presented by DNA st...Continue Reading

Citations

Mar 22, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Liping ChenMancang Gu

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