FABRICA: A Bioreactor Platform for Printing, Perfusing, Observing, & Stimulating 3D Tissues

Scientific Reports
Lester J SmithBurcin Ekser

Abstract

We are introducing the FABRICA, a bioprinter-agnostic 3D-printed bioreactor platform designed for 3D-bioprinted tissue construct culture, perfusion, observation, and analysis. The computer-designed FABRICA was 3D-printed with biocompatible material and used for two studies: (1) Flow Profile Study: perfused 5 different media through a synthetic 3D-bioprinted construct and ultrasonically analyzed the flow profile at increasing volumetric flow rates (VFR); (2) Construct Perfusion Study: perfused a 3D-bioprinted tissue construct for a week and compared histologically with a non-perfused control. For the flow profile study, construct VFR increased with increasing pump VFR. Water and other media increased VFR significantly while human and pig blood showed shallow increases. For the construct perfusion study, we confirmed more viable cells in perfused 3D-bioprinted tissue compared to control. The FABRICA can be used to visualize constructs during 3D-bioprinting, incubation, and to control and ultrasonically analyze perfusion, aseptically in real-time, making the FABRICA tunable for different tissues.

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Citations

Jun 7, 2018·American Journal of Public Health·Jonathan M Metzl
Dec 4, 2019·Biotechnology and Bioengineering·T J SegoLester Jeshua Smith
Aug 25, 2020·Current Opinion in Organ Transplantation·Malcolm MacConmaraParsia A Vagefi
Aug 7, 2019·Current Opinion in Organ Transplantation·Ping LiBurcin Ekser
Mar 15, 2020·Xenotransplantation·Angela M Chen, Christopher Burlak
Dec 1, 2020·PloS One·Marius GenslerJan Hansmann
Jan 22, 2021·Journal of Materials Science. Materials in Medicine·Benjamin LangridgePeter E Butler
Apr 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Tarun AgarwalTapas Kumar Maiti
Aug 15, 2021·Advances in Surgery·Malcolm MacConmara, Parsia A Vagefi

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Methods Mentioned

BETA
bioprinter
imaging techniques
bioprinting

Software Mentioned

PreForm
AutoDesk Inventor Aided Design CAD
Leica Application
AutoDesk
FABRICA
Aixplorer
EnvisionTec
Regenova
Bio 3D Designer

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