Fabricating an Amperometric Cholesterol Biosensor by a Covalent Linkage between Poly(3-thiopheneacetic acid) and Cholesterol Oxidase.

Sensors
Po-Chin NienKuo-Chuan Ho

Abstract

In this study, use of the covalent enzyme immobilization method was proposed to attach cholesterol oxidase (ChO) on a conducting polymer, poly(3-thiopheneacetic acid), [poly(3-TPAA)]. Three red-orange poly(3-TPAA) films, named electrodes A, B and C, were electropolymerized on a platinum electrode by applying a constant current of 1.5 mA, for 5, 20 and 100 s, respectively. Further, 1-ethyl-3-(3-dimethylamiopropyl)carbodiimide hydrochloride (EDC · HCl) and N-hydroxysuccinimide (NHS) were used to activate the free carboxylic groups of the conducting polymer. Afterwards, the amino groups of the cholesterol oxidase were linked on the activated groups to form peptide bonds. The best sensitivity obtained for electrode B is 4.49 mA M(-1) cm(-2), with a linear concentration ranging from 0 to 8 mM, which is suitable for the analysis of cholesterol in humans. The response time (t(95)) is between 70 and 90 s and the limit of detection is 0.42 mM, based on the signal to noise ratio equal to 3. The interference of species such as ascorbic acid and uric acid increased to 5.2 and 10.3% of the original current response, respectively, based on the current response of cholesterol (100%). With respect to the long-term stability, the sensing respon...Continue Reading

Citations

Nov 28, 2013·Talanta·Saniye SoylemezLevent Toppare
Jul 31, 2013·Colloids and Surfaces. B, Biointerfaces·Saniye SoylemezLevent Toppare
Mar 19, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Murat Ates
Feb 9, 2012·Microbial Biotechnology·J L GarcíaB Galán
Apr 30, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Soorya V C, Sheela Berchmans
Oct 4, 2014·ACS Applied Materials & Interfaces·Saniye SoylemezLevent Toppare
Jun 10, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Anjum GahlautVikas Hooda

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Methods Mentioned

BETA
biosensor
scanning electron microscopy

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