Fabrication and application of heterogeneous printed mouse phantoms for whole animal optical imaging

Applied Optics
Brian Z BentzKevin J Webb

Abstract

This work demonstrates the usefulness of 3D printing for optical imaging applications. Progress in developing optical imaging for biomedical applications requires customizable and often complex objects for testing and evaluation. There is therefore high demand for what have become known as tissue-simulating "phantoms." We present a new optical phantom fabricated using inexpensive 3D printing methods with multiple materials, allowing for the placement of complex inhomogeneities in complex or anatomically realistic geometries, as opposed to previous phantoms, which were limited to simple shapes formed by molds or machining. We use diffuse optical imaging to reconstruct optical parameters in 3D space within a printed mouse to show the applicability of the phantoms for developing whole animal optical imaging methods. This phantom fabrication approach is versatile, can be applied to optical imaging methods besides diffusive imaging, and can be used in the calibration of live animal imaging data.

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Citations

Feb 1, 2017·Radiation Research·David WelchDavid J Brenner
Sep 6, 2018·Physics in Medicine and Biology·Haozhao ZhangJianfeng Qiu
Nov 30, 2018·Medical Physics·Nolan EsplenMagdalena Bazalova-Carter
Aug 29, 2019·Medical Physics·Nolan EsplenMagdalena Bazalova-Carter
Nov 15, 2016·Optics Letters·Brian Z BentzKevin J Webb
Sep 11, 2020·International Journal of Radiation Biology·Andrea L DiCarloDavid R Cassatt
Jul 1, 2017·Biomedical Optics Express·Laura A DempseyJeremy C Hebden
Sep 28, 2018·Biomedical Optics Express·Yi LiuT Joshua Pfefer
May 15, 2021·Optics Express·Brian Z BentzJeremy B Wright

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