Fabrication and biological evaluation of uniform extracellular matrix coatings on discontinuous photolithography generated micropallet arrays.

Journal of Biomedical Materials Research. Part a
Nicholas M GunnEdward L Nelson

Abstract

The recent identification of rare cell populations within tissues that are associated with specific biological behaviors, for example, progenitor cells, has illuminated a limitation of current technologies to study such adherent cells directly from primary tissues. The micropallet array is a recently developed technology designed to address this limitation by virtue of its capacity to isolate and recover single adherent cells on individual micropallets. The capacity to apply this technology to primary tissues and cells with restricted growth characteristics, particularly adhesion requirements, is critically dependent on the capacity to generate functional extracellular matrix (ECM) coatings. The discontinuous nature of the micropallet array surface provides specific constraints on the processes for generating the desired ECM coatings that are necessary to achieve the full functional capacity of the micropallet array. We have developed strategies, reported herein, to generate functional coatings with various ECM protein components: fibronectin, EHS tumor basement membrane extract, collagen, and laminin-5; confirmed by evaluation for rapid cellular adherence of four dissimilar cell types: fibroblast, breast epithelial, pancreatic...Continue Reading

References

Jun 1, 1991·The Journal of Cell Biology·J E Schwarzbauer
Dec 1, 1986·European Journal of Biochemistry·K SkorstengaardS Magnusson
Mar 1, 1972·The Review of Scientific Instruments·W A BonnerL A Herzenberg
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T E PetersenS Magnusson
Oct 6, 1999·Current Opinion in Cell Biology·J E Schwarzbauer, J L Sechler
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·G Baneyx, V Vogel
Apr 25, 2003·Experimental and Molecular Pathology·Sanford H Barsky
Jan 12, 2005·Breast Cancer Research : BCR·Mary Helen Barcellos-Hoff, Daniel Medina
Aug 3, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Yong Mao, Jean E Schwarzbauer
Sep 9, 2005·Nature Reviews. Cancer·Thomas BrabletzThomas Kirchner
Jan 18, 2006·Cellular and Molecular Life Sciences : CMLS·L Kopfstein, G Christofori
Mar 8, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Yuli WangNancy L Allbritton
Apr 28, 2006·BMC Genomics·Asa J OudesAlvin Y Liu
May 3, 2006·Cancer Research·Linheng Li, William B Neaves
Sep 6, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Yuli WangNancy L Allbritton
Sep 7, 2006·Analytical and Bioanalytical Chemistry·Christopher E SimsNancy L Allbritton
Sep 27, 2006·Handbook of Experimental Pharmacology·L Nikitenko, C Boshoff
Oct 31, 2006·The Journal of Investigative Dermatology. Symposium Proceedings·Kim S MidwoodJean E Schwarzbauer
Jan 5, 2007·Molecular and Cellular Biochemistry·Souichi FuruhataShumpei Ohnami
Jan 16, 2007·Analytical Chemistry·Georgina To'a SalazarNancy L Allbritton
Feb 10, 2007·Analytical Chemistry·Yuli WangNancy L Allbritton
Oct 24, 2007·Analytical Chemistry·Jeng-Hao PaiNancy L Allbritton
Jan 30, 2008·Current Protocols in Cell Biology·Iwona Wierzbicka-PatynowskiJean E Schwarzbauer
Jul 8, 2008·Journal of Biomedical Optics·Georgina To'a SalazarNancy L Allbritton
Nov 27, 2008·Cell and Tissue Research·Xiaobing Fu, Haihong Li
Jan 28, 2009·Nature Reviews. Immunology·Antonio UccelliVito Pistoia
Feb 13, 2009·Journal of Cellular and Molecular Medicine·J García-CastroP Menendez

❮ Previous
Next ❯

Citations

Dec 14, 2011·Analytical and Bioanalytical Chemistry·David A DetwilerNancy L Allbritton
Feb 26, 2014·Journal of Membrane Science·Monica Y KimElliot E Hui
Nov 26, 2015·Lab on a Chip·Wesley A Cox-MuranamiMark Bachman
Oct 14, 2017·Scientific Reports·Trisha M WesterhofEdward L Nelson
Oct 26, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Nicholas M GunnEdward L Nelson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.