Fabrication and characterization of nano-fibrous bilayer composite for skin regeneration application

Methods : a Companion to Methods in Enzymology
Shaghayegh ArastehSahba Mobini

Abstract

Full thickness wound healing with minimal scarring and complete restoration of normal skin properties still remains as a clinical challenge. In this study, a bilayer skin substitute has been fabricated to biomimic the microstructure of natural extracellular matrix of the skin. Human amniotic membrane (HAM) and silk fibroin nano-fibers were combined to produce bilayer construct, which was further treated and characterized. HAM was obtained from healthy mothers and de-epithelized by means of fine enzymatic method to preserve the extracellular structure. Fibroin protein was extracted from fresh Bombyx mori cocoons and transformed to uniform nano-fiberous structure, which was used as a coating layer on the de-epithelized membrane. Surface modification through oxygen plasma treatment was attempted to further induce hydrophilicity. Subsequently, scaffolds were fully characterized in terms of morphology, mechanical properties, hydrophilicity and cell culture response. Histological and immunohistological staining demonstrated localization of fibronectin, cell denudation and structural integrity of HAM after de-epithelization. Scanning electron microscopy images showed bead-free silk fibroin nano-fibers with the average diameter of 250n...Continue Reading

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Citations

Apr 14, 2016·Methods : a Companion to Methods in Enzymology·Hooman Sadri-Ardekani, Anthony Atala
Jun 20, 2017·Differentiation; Research in Biological Diversity·Ahmed T El-SerafiFolke Sjöberg
Jun 26, 2018·Journal of Functional Biomaterials·Daniela A FrauchigerBenjamin Gantenbein
Jul 2, 2019·The International Journal of Lower Extremity Wounds·Fahimeh RezaieHojjat Naderi-Meshkin
Jun 11, 2020·Molecular Pharmaceutics·Shahriar SharifiMorteza Mahmoudi
Nov 17, 2020·Frontiers in Cardiovascular Medicine·Dillon K JarrellJeffrey G Jacot
Aug 11, 2019·The Journal of Surgical Research·Shaghayegh ArastehSomaieh Kazemnejad
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Hee Jung ChoiJae Yong Han

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