Fabrication and modification of implantable optrode arrays for in vivo optogenetic applications

Biophysical Reports
Lulu WangYi Lu

Abstract

Recent advances in optogenetics have established a precisely timed and cell-specific methodology for understanding the functions of brain circuits and the mechanisms underlying neuropsychiatric disorders. However, the fabrication of optrodes, a key functional element in optogenetics, remains a great challenge. Here, we report reliable and efficient fabrication strategies for chronically implantable optrode arrays. To improve the performance of the fabricated optrode arrays, surfaces of the recording sites were modified using optimized electrochemical processes. We have also demonstrated the feasibility of using the fabricated optrode arrays to detect seizures in multiple brain regions and inhibit ictal propagation in vivo. Furthermore, the results of the histology study imply that the electrodeposition of composite conducting polymers notably alleviated the inflammatory response and improved neuronal survival at the implant/neural-tissue interface. In summary, we provide reliable and efficient strategies for the fabrication and modification of customized optrode arrays that can fulfill the requirements of in vivo optogenetic applications.

References

Mar 24, 2006·Journal of Neuroscience Methods·Longnian LinJoe Z Tsien
Oct 4, 2006·Neuron·Andrew B SchwartzDaniel W Moran
Apr 6, 2007·Nature·Feng ZhangKarl Deisseroth
Apr 24, 2008·Annual Review of Biomedical Engineering·Stuart F Cogan
Mar 21, 2009·Science·Viviana GradinaruKarl Deisseroth
Apr 30, 2009·Annual Review of Biomedical Engineering·Warren M GrillRavi V Bellamkonda
Jul 13, 2011·Neuron·Ofer YizharKarl Deisseroth
Dec 6, 2011·Nature Neuroscience·Polina AnikeevaKarl Deisseroth
Jun 15, 2013·Biomedical Microdevices·Evon S EreifejPamela J VandeVord
Jul 26, 2013·Journal of Neural Engineering·Sanyuan ChenHongda Chen
Nov 30, 2013·Journal of Neuroscience Methods·Cheng ZhongLiping Wang
Mar 4, 2015·Nature·Nuo LiKarel Svoboda
Jun 10, 2016·Nature·Philip TovoteAndreas Lüthi
Aug 4, 2016·Nature·Christopher A ZimmermanZachary A Knight
Feb 16, 2017·Journal of Neural Engineering·Ege Iseri, Duygu Kuzum
May 4, 2017·Nature·L Ian SchmittMichael M Halassa
Jan 6, 2018·Journal of Neural Engineering·Fahimeh DehkhodaPatrick Degenaar

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Citations

Sep 5, 2019·AAPS PharmSciTech·Kawther Khalid AhmedAliasger K Salem
Mar 11, 2020·Frontiers in Neuroscience·Amisha A PatelShuzo Sakata
Oct 24, 2020·Journal of Neuroscience Methods·Sanaya K Stocke, Chad L Samuelsen
Jun 29, 2021·Journal of Micromechanics and Microengineering : Structures, Devices, and Systems·Zeinab RamezaniHui Fang

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Methods Mentioned

BETA
dissection
scanning electron microscopy

Software Mentioned

MATLAB
SPSS
NeuroExplorer

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