Fabrication of a Corneal Model Composed of Corneal Epithelial and Endothelial Cells via a Collagen Vitrigel Membrane Functioned as an Acellular Stroma and Its Application to the Corneal Permeability Test of Chemicals

Drug Metabolism and Disposition : the Biological Fate of Chemicals
Hiroyuki Yamaguchi, Toshiaki Takezawa

Abstract

A collagen vitrigel membrane (CVM) we developed can function as both a scaffold for cells and a pathway for chemicals. To extrapolate the corneal permeability of chemicals in vivo, we proposed six corneal models using the CVM. Thin and thick CVMs were used as models for Bowman's membrane (BM) and an acellular stroma (AS), respectively. Models for a corneal epithelium (CEpi), a CEpi-AS, a CEpi-endothelium (Endo), and a CEpi-AS-Endo were fabricated by culturing corneal epithelial cells and/or corneal endothelial cells on the surface of CVMs. Subsequently, the permeability coefficient (Papp) value of each model was calculated using five chemicals with different molecular radii; cyanocobalamin and four fluorescein isothiocyanate-dextrans (FD) (FD-4, FD-10, FD-20, and FD-40). The slopes of Papp versus molecular radii of those chemicals in the both BM and AS models were almost similar to data using an excised rabbit corneal stroma. The ratios of Papp values in models for BM, CEpi, and CEpi-Endo against those in data using an excised rabbit cornea were calculated as 75.4-fold, 6.4-fold, and 4.5-fold for FD-4, and 38.7-fold, 10.0-fold, and 4.2-fold for FD-10, respectively. Similarly, those in models for AS, CEpi-AS, and CEpi-AS-Endo we...Continue Reading

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Citations

Oct 20, 2018·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Aarti Sawant-Basak, R Scott Obach
Aug 21, 2020·In Vitro Cellular & Developmental Biology. Animal·Ai ShimaShoji Takeuchi
Feb 28, 2019·Journal of Tissue Engineering and Regenerative Medicine·Hideaki OkuyamaKoichi Omori
Feb 6, 2021·In Vitro Cellular & Developmental Biology. Animal·Yulia Kaluzhny, Mitchell Klausner
Dec 19, 2020·Pharmaceutics·Laura García-Posadas, Yolanda Diebold
Nov 10, 2020·Experimental Eye Research·Jorge L Alió Del BarrioJorge L Alió
Jun 9, 2021·Experimental Eye Research·Rodi AbdalkaderKen-Ichiro Kamei
Aug 25, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Rifa WidyaningrumTsung-Jen Wang

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