Fabrication of a phospholipid membrane-mimetic film on the luminal surface of an ePTFE vascular graft

Biomaterials
Sumanas W JordanElliot L Chaikof

Abstract

A stabilized, membrane-mimetic film was produced on the luminal surface of an ePTFE vascular graft by in situ photopolymerization of an acrylate functonalized phospholipid using a fiber optic diffusing probe. The phospholipid monomer was synthesized, prepared as unilamellar vesicles, and fused onto close-packed octadecyl chains that were components of an amphiphilic terpolymer anchored onto the polyelectrolyte multilayer (PEM) by electrostatic interactions. Scanning electron microscopy (SEM) confirmed that gelatin impregnation of the graft followed by the subsequent biomimetic film coating filled in the fibril and node structure of the luminal surface of the ePTFE graft and was smooth. The lipid film displayed an initial advancing contact angle of 44 degrees , which increased to 55 degrees after being subjected to a wall shear rate of 500s(-1) for 24h at 37 degrees C in phosphate buffered saline (PBS). Fourier transform (FT-IR) spectroscopy was used to characterize the stages of biomimetic film assembly and confirmed the stability of the film under shear flow conditions. In vivo assessment using a baboon femoral arteriovenous shunt model demonstrated minimal platelet and fibrinogen deposition over a 1-h blood-contacting period....Continue Reading

References

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Citations

Mar 28, 2009·Journal of Biomaterials Science. Polymer Edition·Yingjun WangQichun Zhao
Apr 13, 2013·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Robert VlachovskyTimothy McGloughlin
Jun 7, 2011·Annual Review of Biomedical Engineering·Song Li, Jeffrey J D Henry
Oct 27, 2015·Annals of Biomedical Engineering·Keith M FaucherElazer R Edelman
Sep 16, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Settimio PacelliArghya Paul
Dec 20, 2011·Advanced Functional Materials·Zheng QuElliot L Chaikof
Jul 8, 2015·Journal of Thrombosis and Haemostasis : JTH·I H JafferJ I Weitz
Aug 8, 2007·Journal of Vascular Surgery·Sumanas W Jordan, Elliot L Chaikof
Apr 9, 2008·Circulation·Muneera R KapadiaMelina R Kibbe

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