Fabrication of Bio-Based Gelatin Sponge for Potential Use as A Functional Acellular Skin Substitute

Polymers
Mior Muhammad Amirul ArifMohd Heikal Mohd Yunus

Abstract

Gelatin possesses biological properties that resemble native skin and can potentially be fabricated as a skin substitute for full-thickness wound treatment. The native property of gelatin, whereby it is easily melted and degraded at body temperature, could prevent its biofunctionality for various applications. This study aimed to fabricate and characterise buffalo gelatin (Infanca halal certified) crosslinked with chemical type crosslinker (genipin and genipin fortified with EDC) and physicaly crosslink using the dihydrothermal (DHT) method. A porous gelatin sponge (GS) was fabricated by a freeze-drying process followed by a complete crosslinking via chemical-natural and synthetic-or physical intervention using genipin (GNP), 1-ethyl-3-(3-dimethylaminopropyl) (EDC) and dihydrothermal (DHT) methods, respectively. The physicochemical, biomechanical, cellular biocompatibility and cell-biomaterial interaction of GS towards human epidermal keratinocytes (HEK) and dermal fibroblasts (HDF) were evaluated. Results showed that GS had a uniform porous structure with pore size ranging between 60 and 200 µm with high porosity (>78.6 ± 4.1%), high wettability (<72.2 ± 7.0°), high tensile strain (>13.65 ± 1.10%) and 14 h of degradation rate....Continue Reading

References

Oct 1, 1992·The Journal of Investigative Dermatology·T LearyM Stanley
Feb 9, 2000·American Journal of Physiology. Renal Physiology·L MicheaM B Burg
Nov 19, 2010·Journal of Biomaterials Science. Polymer Edition·Mina MekhailWankei Wan
May 16, 2013·Tissue Engineering. Part B, Reviews·Qiu Li Loh, Cleo Choong
Dec 7, 2013·Biomedical Engineering Online·Chuangang YouChunmao Han
Mar 20, 2015·Macromolecular Bioscience·Sofia AmadoriAdriana Bigi
Apr 19, 2015·Trends in Biotechnology·Narendra ReddyQiuran Jiang
Jun 21, 2015·Cytotechnology·Ieva BružauskaitėEiva Bernotienė
Sep 27, 2015·International Journal of Biological Macromolecules·Wenhang WangYonghao Ni
Mar 24, 2016·Journal of Burn Care & Research : Official Publication of the American Burn Association·Jeremy GovermanBenjamin Levi
May 8, 2016·Cellular and Molecular Life Sciences : CMLS·Mathew N NicholasSaeid Amini-Nik
Aug 16, 2016·Materials Science & Engineering. C, Materials for Biological Applications·M B FauziS R Chowdhury
Oct 1, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Najwa MohamadMohd Cairul Iqbal Mohd Amin
Sep 19, 2017·International Journal of Biological Macromolecules·Ahmad OryanHamed Daemi
Aug 11, 2018·Frontiers in Bioengineering and Biotechnology·Houman SavojiMohsen Akbari
Aug 11, 2018·Molecular Biology Reports·T G Sahana, P D Rekha
Sep 29, 2018·The Journal of Dermatological Treatment·Christina DaiAmor Khachemoune
Oct 26, 2018·Advances in Experimental Medicine and Biology·Shiplu Roy ChowdhuryRuszymah Binti Haji Idrus
Jan 27, 2019·Bioengineering & Translational Medicine·Samson AfewerkiAli Khademhosseini
Feb 25, 2019·International Journal of Pharmaceutics·Forough HafeziJoshua Boateng
Feb 28, 2019·Journal of Tissue Engineering and Regenerative Medicine·Fauzi Mh BusraShiplu R Chowdhury
Feb 8, 2020·Pharmaceutics·Marta NardiniMaddalena Mastrogiacomo
May 28, 2020·Journal of Biomaterials Science. Polymer Edition·Jegadevswari SelvarajahYogeswaran Lokanathan

❮ Previous
Next ❯

Methods Mentioned

BETA
scanning electron microscopy
infrared spectroscopy
X-ray
circumcision
Assay
scintillation spectroscopy

Software Mentioned

Axio Vision LE
Phenom Pro X

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Xunjie JinGongping Xu
International Journal of Biological Macromolecules
Juthamas RatanavarapornSiriporn Damrongsakkul
Materials Science & Engineering. C, Materials for Biological Applications
Elahe EntekhabiArghavan Kazemzadeh
© 2021 Meta ULC. All rights reserved