Fabrication of DNA microarrays on nanoengineered polymeric ultrathin film prepared by self-assembly of polyelectrolyte multilayers

Langmuir : the ACS Journal of Surfaces and Colloids
Xichun ZhouJizhong Zhou

Abstract

Microarray-based technology is in need of flexible and cost-effective chemistry for fabrication of oligonucleotide microarrays. We have developed a novel method for the fabrication of oligonucleotide microarrays with unmodified oligonucleotide probes on nanoengineered three-dimensional thin films that are deposited on glass slides by consecutive layer-to-layer adsorption of polyelectrolytes. Unmodified oligonucleotide probes were spotted and immobilized on these multilayered polyelectrolyte thin films (PET) by electrostatic adsorption and entrapment on the porous structure of the PET film. The PET provides higher probe binding capacity and thus higher hybridization signal than that of the traditional two-dimensional aminosilane and poly-L-lysine coated slides. Immobilized probe densities of 3.4 x 10(12)/cm2 were observed for microarray spots on PET with unmodified 50-mer oligonucleotide probes, which is comparable to the immobilized probe densities of alkyamine-modified 50-mer probes end-tethered on an aldehyde-functionalized slide. The study of hybridization efficiency showed that 90% of immobilized probes on PET film are accessible to target DNA to form duplex format in hybridization. The DNA microarray fabricated on PET film...Continue Reading

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Citations

Aug 10, 2006·Microbial Ecology·T J GentryJ Zhou
May 23, 2012·Chemistry of Materials : a Publication of the American Chemical Society·Adam H BroderickDavid M Lynn
Sep 13, 2014·Chemical Communications : Chem Comm·Yifei ZhangZheng Liu
Apr 13, 2010·Trends in Biotechnology·Seong Woon RohJin-Woo Bae
Jan 13, 2011·Angewandte Chemie·Hyun Ok HamPhillip B Messersmith
Jul 2, 2005·Critical Reviews in Microbiology·Douglas R Call
Mar 30, 2021·Biosensors & Bioelectronics·Madalina Elena CulicaSergiu Coseri
Jun 24, 2008·Analytical Chemistry·Alfredo J IbáñezAles Svatos
Jul 20, 2019·ACS Applied Materials & Interfaces·William J BrittainJürgen Rühe
Nov 15, 2005·Biomacromolecules·Chuan Liang FengHolger Schönherr
Jul 12, 2005·Biomacromolecules·Thomas AmeringerMartin Moeller

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