Fabrication of ordered metallic and magnetic heterostructured DNA-Nanoparticle hybrids

Colloids and Surfaces. B, Biointerfaces
Joseph M Kinsella, Albena Ivanisevic

Abstract

Here we provide a method based on enzymatically catalyzed reactions to cleave and ligate DNA molecules coated with nanoparticles to fabricate multi-component structures. This is done by simultaneously digesting two solutions of nanoparticle coated DNA, one with iron oxide particles the other gold particles, which yields short DNA fragments with complementary single stranded overhangs. When added together and re-attached using ligase enzymes multi-component nanoparticle coated structures are formed providing a novel method to fabricate complicated nanoparticle arrangements from the bottom up. We evaluated the fabrication by characterizing the samples with gel electrophoresis and magnetic force microscopy (MFM). The electrophoresis provides proof that the coated DNA molecules were digested with restriction enzymes and ligated by the T4 ligase enzymes. MFM experiments allow us to visualize the multi-component strands and analyze the magnetic versus metallic segments.

References

Sep 30, 1994·Science·A BensimonD Bensimon
Jun 5, 1995·Physical Review Letters·D BensimonA Bensimon
Dec 9, 2004·Biomaterials·Dorjderem Nyamjav, Albena Ivanisevic
Nov 3, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Gary BraunNorbert O Reich
Feb 24, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Joseph M Kinsella, Albena Ivanisevic

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Citations

Nov 16, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Hamsa JaganathanAlbena Ivanisevic
Mar 9, 2011·ACS Applied Materials & Interfaces·Hamsa JaganathanAlbena Ivanisevic
Aug 21, 2012·Nanoscale·Hasan Daw A MohamedAndrew Houlton

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