Fabrication of Prussian Blue modified ultramicroelectrode for GOD imaging using scanning electrochemical microscopy

Bioelectrochemistry
Jianping Li, Jianguo Yu

Abstract

A Prussian Blue (PB) film modified disk ultramicroelectrode (UME) was fabricated by electrochemical deposition technique on a Pt-disk UME. The electrocatalytical reductions of hydrogen peroxide derived from glucose oxidase (GOD) on this modified UME were investigated. The enzymatic biochemical reactivity was imaged by scanning electrochemical microscopy (SECM) utilizing the PB film modified UME. It is evident that sensitivity and spatial resolution for hydrogen peroxide measurement were improved obviously. SECM images obtained clearly revealed the concentration profile of the reaction products around the enzymes. The PB film modified microelectrode is in the nature of simple preparation, high catalytic activity on hydrogen peroxide and substrate selectivity for SECM etc.

References

Dec 18, 2001·Biotechnology and Bioengineering·T KayaT Matsue
Jun 22, 2005·Biosensors & Bioelectronics·Kuniaki NagamineTomokazu Matsue
Jul 19, 2005·Biosensors & Bioelectronics·Angelika KuengBoris Mizaikoff
Jun 27, 2006·Biosensors & Bioelectronics·Allen J BardWei Zhan

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