Fabrication of reusable electrochemical biosensor and its application for the assay of α-glucosidase activity

Analytica Chimica Acta
Jinlong LiGenxi Li

Abstract

A reusable biosensor has been fabricated in this work for the assay of α-glucosidase activity and the inhibitor screening. In this design, the aptamer of ATP is split as split aptamer 1 (Apt 1) and split aptamer 2 (Apt 2), and Apt 2 can link gold nanoparticles (AuNPs) modified with Apt 1 and 4-aminophenyl-α-d-glucopyranoside (pAPG). Consequently, the functional AuNPs can be immobilized onto the surface of gold electrode, allowing for salt-induced regeneration. In the presence of α-glucosidase, the glycosyl of pAPG is cut off, and the electroactive phenolic hydroxyls appear to give a strong current signal. Furthermore, the biosensor can be recovered very easily by incubating it in water to dissociate the AuNPs modified with Apt 1 and pAPG. So, a new biosensor for α-glucosidase activity detection and inhibitor screening is developed based on enzyme-activated signal generation and recovery. The biosensor may also exhibit good sensitivity for α-glucosidase determination with the detection limit 0.005 U/mL and can be reused by water-washing regeneration with good repeatability. Meanwhile this biosensor can also be utilized for inhibitor screening, which may have potential for clinical applications.

Citations

Mar 1, 2020·Nucleic Acids Research·Mégane DebiaisSabine Müller
Feb 23, 2020·Current Medicinal Chemistry·Minmin ChangJunli Ren
Dec 31, 2020·Biomedicines·Intan Rosalina SuhitoTae-Hyung Kim
Apr 22, 2019·Analytica Chimica Acta·Morteza Alinezhad NameghiSeyed Mohammad Taghdisi
Jun 17, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Xucan YuanZhili Xiong
Aug 20, 2019·ACS Applied Materials & Interfaces·Yingying ZhongSuqing Zhao

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