Fabrication of single gel with different mechanical stiffness using three-dimensional mold

Journal of Biomedical Materials Research. Part a
Gulsan Ara Sathi KaziTakuya Matsumoto

Abstract

The hydrogel is considering as functional substrates for three-dimensional (3D) environment mimicking the native tissue in vitro. To get the cell or tissue culture result in different stiffness, researchers are used separate gel at the different time. Sometimes these results are manipulated by surrounding environment. To overcome this, we prepared a single hydrogel with different young modulus using a 3D printed mold and cell culture and tissue culture was performed to check the functional capacity. In this proposed device we successfully produced a multi properties agarose hydrogel on a single platform. We designed different mold pattern to confirm that this gel formation technique can be used for any types of design and many different concentrated hydrogels can be combined together. The cell and tissue culture results showed that even in same planer surface, each gel solely maintains their own physical properties and control the cell and tissue adherence and proliferation behavior. The protocol is fairly simple and reproducible. The design helps to produce consistent gel thickness, shape, and size. This 3D mold has provided a new way to study the fundamental cellular responses to engineered microenvironments that may have a h...Continue Reading

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Citations

Aug 3, 2019·Macromolecular Bioscience·Aurica P ChiriacVlad Mihai Chiriac
Jan 23, 2020·Journal of Functional Biomaterials·Yueh-Hsun Kevin YangGilda A Barabino

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