Fabrication of the FGF1-functionalized sericin hydrogels with cell proliferation activity for biomedical application using genetically engineered Bombyx mori (B. mori) silk

Acta Biomaterialia
Feng WangQingyou Xia

Abstract

Sericin, as the major component of Bombyx mori silk, is a useful biomaterial for tissue engineering due to its hydrophilicity, biocompatibility and biodegradability. Here, we report the fabrication of a human acidic fibroblast growth factor (FGF1)-functionalized sericin hydrogel using a transgenic silkworm spun silk with FGF1 incorporated in its sericin layer. Sericin, together with FGF1, were simultaneously extracted from the silk fiber and then exposed to cold-induced hydrogel formation without additional crosslinking. The fabricated FGF1 sericin hydrogels demonstrated injectability, useful mechanical properties and a porous microstructure, which contributed to cell adhesion and survival. In addition, FGF1 achieved long-term storage in the sericin hydrogels over a wide range of temperatures. Further, the sericin-FGF1 demonstrated sustained release to promote cell proliferation and wound healing. Furthermore, cellular inflammatory responses showed that the FGF1 sericin hydrogels exhibited biocompatibility and no immunogenicity. This study revealed the successful exploration of FGF1-functionalized sericin hydrogels as a new protein-based biomaterial to expand applications of FGF1 and sericin in tissue and medical engineering. F...Continue Reading

Citations

Dec 24, 2018·Biomedical Chromatography : BMC·Fan SunTao Fan
Nov 30, 2020·Acta Biomaterialia·Feng WangDavid L Kaplan
Dec 6, 2019·Acta Biomaterialia·Dimple Chouhan, Biman B Mandal
Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Amir JafariBehnaz Ashtari
Feb 10, 2021·Colloids and Surfaces. B, Biointerfaces·Xinyi LiZhigang Xu
Apr 23, 2020·Biomacromolecules·Christopher S FoxSusan Pedigo
Mar 18, 2021·ACS Biomaterials Science & Engineering·Sara Baptista-SilvaAna Leite Oliveira
Jun 10, 2019·ACS Biomaterials Science & Engineering·Yingjie HangDavid L Kaplan
Jun 14, 2020·Current Pharmaceutical Design·Rasika SuryawanshiShubhini A Saraf
Jul 10, 2019·Biomacromolecules·Jason L GuoAntonios G Mikos
Sep 14, 2021·Biomacromolecules·Hoyoung LeeWon Jun Lee
Dec 9, 2021·Archives of Insect Biochemistry and Physiology·Shanshan TaoChongjun Ye

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biomaterial Engineering

Advances in biomaterial engineering have permitted the development of sophisticated drug-releasing materials with a biomimetic 3D support that allow a better control of the microenvironment of transplanted cells. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.