Fabrication of three-dimensional multi-protein microstructures for cell migration and adhesion enhancement

Biomedical Optics Express
Yong Da SieShean-Jen Chen

Abstract

In this study, three-dimensional (3D) multi-component microstructures were precisely fabricated via multiphoton excited photochemistry using a femtosecond laser direct-writing system with proposed repetition positioning and vector scanning techniques. Extracellular matrix (ECM) proteins, such as fibronectin (FN), are difficult to stack and form 3D structures larger than several-hundred microns in height due to the nature of their protein structure. Herein, to fabricate complex 3D microstructures with FN, a 3D scaffold was designed and formed from bovine serum albumin (BSA), after which human FN was inserted at specific locations on the BSA scaffold; in this manner, the fabricated ECM microstructure can guide cells in a 3D environment. A human breast cancer cell line, MDA-MB-231, was used to investigate the behavior of cell migration and adhesion on the fabricated human FN and BSA protein structures. Experimental results indicate that many cells are not able to attach or climb on a 3D structure's inclined plane without FN support; hence, the influence of cell growth in a 3D context with FN should being taken into consideration. This 3D multi-protein fabrication technique holds potential for cell studies in designed complex 3D EC...Continue Reading

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Citations

Jan 24, 2017·IEEE Transactions on Bio-medical Engineering·Se-Jun LeeLijie Grace Zhang
Feb 23, 2019·Advanced Materials·Marc HipplerMartin Bastmeyer
Jun 19, 2019·Lab on a Chip·Andrew C LamontRyan D Sochol
Aug 14, 2019·Small·Marco Carlotti, Virgilio Mattoli
Jun 6, 2017·Angewandte Chemie·Christopher Barner-KowollikMartin Wegener
Jul 14, 2018·Scientific Reports·Yong Da SieShean-Jen Chen
Jan 20, 2021·ACS Biomaterials Science & Engineering·Daniela Serien, Koji Sugioka
Mar 13, 2017·ACS Biomaterials Science & Engineering·Daniela Serien, Shoji Takeuchi
Sep 7, 2020·Biophysical Journal·Yongrou ZhangXuefeng Zhou

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