Fabrication of tissue engineered tympanic membrane patches using computer-aided design and injection molding

The Laryngoscope
Morgan E HottLawrence J Bonassar

Abstract

The goal of the current study was to use computer-aided design and injection molding technologies to tissue engineer precisely shaped cartilage in the shape of butterfly tympanic membrane patches out of chondrocyte-seeded calcium alginate gels. Molds were designed on SolidWorks 2000 and built out of acrylonitrile butadiene styrene (ABS) using fused deposition modeling (FDM). Tympanic membrane patches were fabricated using bovine articular chondrocytes seeded at 50 x 10 cells/mL in 2% calcium alginate gels. Molded patches were cultured in vitro for up to 10 weeks and assessed biochemically, morphologically, and histologically. Unmolded patches demonstrated outstanding dimensional fidelity, with a volumetric precision of at least 3 microL, and maintained their shape well for up to 10 weeks of in vitro culture. Glycosaminoglycan and collagen content increased steadily over 10 weeks in culture, demonstrating continual deposition of new extracellular matrix consistent with new tissue development. The use of computer-aided design and injection molding technologies allows for the fabrication of very small, precisely shaped chondrocyte-seeded calcium alginate structures that faithfully maintain their shape during in vitro culture. In v...Continue Reading

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Citations

Jun 15, 2006·Tissue Engineering·Daniel L CohenLawrence J Bonassar
Jul 3, 2008·Tissue Engineering. Part a·Jeffery J BallynsLawrence J Bonassar
Jun 22, 2011·Tissue Engineering. Part a·Jangho KimJong Hoon Chung
Oct 4, 2012·Tissue Engineering. Part B, Reviews·Bing Mei TehMarcus D Atlas
Oct 1, 2009·Tissue Engineering. Part C, Methods·Jeffrey J BallynsLawrence J Bonassar
Sep 9, 2010·Tissue Engineering. Part C, Methods·Daniel L CohenLawrence J Bonassar
Oct 25, 2012·Tissue Engineering. Part a·Yi ShenRobert Jeffery Marano
Apr 14, 2012·International Journal of Pediatric Otorhinolaryngology·J Tahar Aissa, M Hultcrantz
Mar 27, 2007·Biomaterials·Christopher S D LeeLawrence J Bonassar
Jun 24, 2015·Scientific Reports·Seung Won KimYun-Hoon Choung
Jul 9, 2009·Journal of Cellular and Molecular Medicine·Jeffrey J Ballyns, Lawrence J Bonassar
Sep 7, 2007·Journal of Biomedical Materials Research. Part a·Jason P GleghornLawrence J Bonassar
Dec 1, 2012·Biotechnology Progress·Jillian M BrennerStephen D Waldman
May 28, 2016·Journal of medical and biological engineering·Eva SchmelzerJörg C Gerlach
Apr 7, 2010·Biomaterials·Christopher S D LeeZvi Schwartz
Dec 3, 2014·Acta otorrinolaringológica española·Leandro RibeiroArtur Condé
May 29, 2008·Clinical Orthopaedics and Related Research·EunHee HanRobert L Sah
Mar 24, 2010·Journal of Biomaterials Applications·Reza GhassemifarTraian V Chirila
Mar 1, 2017·Biofabrication·Almoatazbellah YoussefPaul D Dalton
Sep 5, 2020·Journal of Biomaterials Science. Polymer Edition·Chen LiuHongguang Xu
Nov 10, 2020·Tissue Engineering. Part B, Reviews·Mina AleemardaniAlexander Seifalian

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