Fabrication of TPGS-Stabilized Liposome-PLGA Hybrid Nanoparticle Via a New Modified Nanoprecipitation Approach: In Vitro and In Vivo Evaluation

Pharmaceutical Research
Mengyuan ZhangJianping Liu

Abstract

In this study, a new modified nanoprecipitation approach that more efficient and simpler than conventional approach was developed to synthesize D-alpha-Tocopheryl polyethylene glycol 1000 succinate stabilized liposome-PLGA hybrid nanoparticle, loaded with simvastatin (ST-TLPN). The optimum formulation was screened via investigation of the impact of TPGS mass within polymeric core and lipid shell on the physicochemical properties of nanoparticles respectively. FTIR, and drug release of ST-TLPN were also systematically determined. Finally, the cellular internalization was evaluated using the murine macrophage cell line, in vivo pharmacokinetic behavior and antiatherogenic efficacies were elaborately examined in atherosclerotic rabbit models. With the weight ratio of TPGS-to-PLGA in organic phase of 30% and TPGS-to-lipid in aqueous phase of 35%, ST-TLPN exhibited core-shell structure, sub-100 nm size, EE% of over 90% and a slow release profile. The excellent cellular uptake was displayed in RAW264.7 cell line. Improved pharmacokinetic behavior, and enhanced antiatherogenic efficacy of ST-TLPN in the model animals were also revealed compared with ST-loaded PLGA nanoparticles. These findings suggest the modified nanoprecipitation me...Continue Reading

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Citations

Nov 7, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Darya A KuznetsovaLucia Ya Zakharova
Jan 9, 2020·Chemistry : a European Journal·Tingting JiangRui Guo
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Divesha EssaViness Pillay
Dec 29, 2020·Colloids and Surfaces. B, Biointerfaces·Muhammad Asim FarooqBo Wang
Apr 22, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xinlong ZangXuehong Chen

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Methods Mentioned

BETA
nanoprecipitation
dynamic light scattering
atomic
chip
flow cytometry
AFM
Infrared Spectroscopy

Software Mentioned

Solution
ST
Image
Pro Plus

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