Fabrication of Troponin I Biosensor Composed of Multi-Functional DNA Structure/Au Nanocrystal Using Electrochemical and Localized Surface Plasmon Resonance Dual-Detection Method

Nanomaterials
Taek LeeChulhwan Park

Abstract

In the present study, we fabricated a dual-mode cardiac troponin I (cTnI) biosensor comprised of multi-functional DNA (MF-DNA) on Au nanocrystal (AuNC) using an electrochemical method (EC) and a localized surface plasmon resonance (LSPR) method. To construct a cTnI bioprobe, a DNA 3 way-junction (3WJ) was prepared to introduce multi-functionality. Each DNA 3WJ arm was modified to possess a recognition region (Troponin I detection aptamer), an EC-LSPR signal generation region (methylene blue: MB), and an anchoring region (Thiol group), respectively. After an annealing step, the multi-functional DNA 3WJ was assembled, and its configuration was confirmed by Native-TBM PAGE for subsequent use in biosensor construction. cTnI was also expressed and purified for use in biosensor experiments. To construct an EC-LSPR dual-mode biosensor, AuNCs were prepared on an indium-tin-oxide (ITO) substrate using an electrodeposition method. The prepared multi-functional (MF)-DNA was then immobilized onto AuNCs by covalent bonding. Field emission scanning electron microscope (FE-SEM) and atomic force microscopy (AFM) were used to analyze the surface morphology. LSPR and electrochemical impedance spectroscopy (EIS) experiments were performed to conf...Continue Reading

References

Mar 11, 2010·Advanced Materials·Taek LeeJeong-Woo Choi
Mar 20, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Jin-Ho LeeJeong-Woo Choi
Nov 6, 2013·Cardiology Clinics·Ramesh S KuttyNarain Moorjani
Mar 29, 2014·Advanced Materials·Manal M Y A AlsaifKourosh Kalantar-zadeh
Jul 26, 2014·Talanta·Zeynep AltintasIbtisam E Tothill
Dec 3, 2014·Trends in Cardiovascular Medicine·Puja K MehtaNanette K Wenger
Jan 7, 2015·Nano Letters·Yichao WangKourosh Kalantar-Zadeh
Oct 27, 2015·Biosensors & Bioelectronics·Kihyun KimJeong-Soo Lee
Dec 2, 2015·Biomedical Reports·Sabesan Mythili, Narasimhan Malathi
Jan 1, 2014·Plasmonics·Martin BauchJakub Dostalek
Apr 7, 2017·Scientific Reports·Md AshaduzzamanAshutosh Tiwari
Apr 13, 2017·Heart Views : the Official Journal of the Gulf Heart Association·Rachel Hajar
May 13, 2018·Biosensors·T Joshua MooreBhavya Sharma
Jun 22, 2018·Photodiagnosis and Photodynamic Therapy·V P S JesusM L Castilho
Mar 21, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Taek LeeJunhong Min

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Methods Mentioned

BETA
biosensors
surface plasmon resonance
biosensor
PCR
scanning electron microscopy
AFM

Software Mentioned

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