PMID: 11339991May 8, 2001Paper

Fabrication of ultra-thin polypyrrole-glucose oxidase film from supporting electrolyte-free monomer solution for potentiometric biosensing of glucose

Biosensors & Bioelectronics
S B Adeloju, A N Moline

Abstract

A simple electropolymerisation process is described for the fabrication of an ultra-thin ( approximately 55 nm) polypyrrole (PPy)-glucose oxidase (GOD) film in a supporting electrolyte-free monomer solution for potentiometric biosensing of glucose. The optimum conditions for growing the ultra-thin film include 0.1 M pyrrole, 55-110 U/ml GOD, an applied current density of 0.05 mA/cm(2) and an electrical charge of 25 mC/cm(2). Long-term storage of the biosensor in acetate buffer improved the sensitivity of the biosensor by a factor of approximately two. The biosensor can also be used repeatedly for over 2 months with little or no loss in sensitivity. The interference effect of ascorbic acid was successfully reduced by inclusion of an outer PPy-Cl layer.

Citations

Nov 23, 2006·Analytical and Bioanalytical Chemistry·Jeffrey S LenihanLeonidas G Bachas
Oct 31, 2008·Talanta·Jorge Rubio-RetamaBeatriz López-Ruiz
Feb 27, 2003·Biosensors & Bioelectronics·Lian CenE T Kang
Jul 2, 2003·Biosensors & Bioelectronics·Won Jun Sung, You Han Bae
Jan 10, 2014·Talanta·Abdulazeez T Lawal, Gordon G Wallace
Oct 2, 2012·Biosensors & Bioelectronics·Abdulazeez T Lawal, Samuel B Adeloju
Feb 5, 2013·Physical Chemistry Chemical Physics : PCCP·Jihun Kim, Kyung-Hwa Yoo
Nov 22, 2007·International Journal of Biological Macromolecules·Senem KiralpAtilla Gungor

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