Facile preparation of glycoprotein-imprinted 96-well microplates for enzyme-linked immunosorbent assay by boronate affinity-based oriented surface imprinting

Analytical Chemistry
Xiaodong Bi, Zhen Liu

Abstract

Molecularly imprinted polymers (MIPs), as inexpensive and stable substitutes of antibodies, have shown great promise in immunoassays. Glycoproteins are of significant diagnostic value. To facilitate the application of MIPs in clinical diagnostics, a general and facile imprinting method toward glycoproteins oriented for an enzyme-linked immunosorbent assay (ELISA) in the form of a 96-well microplate is essential but has not been fully explored yet. In this study, a new method called boronate affinity-based oriented surface imprinting was proposed for facile preparation of glycoprotein-imprinted microplates. A template glycoprotein was first immobilized by a boronic acid-modified microplate through boronate affinity binding, and then, a thin layer of polyaniline was formed to cover the microplate surface via in-water self-copolymerization. After the template was removed by an acidic solution, 3D cavities that can rebind the template were fabricated on the microplate surface. Using horseradish peroxidase (HRP) as a model target, the effects of imprinting conditions as well as the properties and performance of the prepared MIPs were investigated. α-Fetoprotein (AFP)-imprinted microplate was then prepared, and thereby, a MIP-based E...Continue Reading

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Citations

Nov 23, 2015·Analytical and Bioanalytical Chemistry·Thomas S Bedwell, Michael J Whitcombe
Dec 19, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wenhua JiLuqi Huang
Sep 29, 2015·Journal of the American Chemical Society·Sudhirkumar ShindeBörje Sellergren
May 9, 2014·Analytical Biochemistry·Brandon Huey-Ping CheongTuck Wah Ng
Jun 4, 2016·Journal of Agricultural and Food Chemistry·Aixiang WangShoufang Xu
Sep 17, 2016·Angewandte Chemie·Jia LiuZhen Liu
Jan 28, 2016·Chemical Communications : Chem Comm·Huan WangYuanjin Zhao
Oct 23, 2015·Chemical Communications : Chem Comm·Danyang YinZhen Liu
Jul 25, 2019·Chemical Communications : Chem Comm·Guiyuan ZhangShouhua Feng
Jan 10, 2020·Expert Review of Proteomics·Wei-Qian CaoPeng-Yuan Yang
Aug 29, 2020·Analytical Methods : Advancing Methods and Applications·Na WangLixia Yang
Oct 1, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Run-Tian MaYan-Ping Shi
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Jan 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiao-Yu SunYan-Ping Shi
Apr 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Run-Tian MaYan-Ping Shi

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