Facile synthesis of near infrared fluorescent trypsin-stabilized Ag nanoclusters with tunable emission for 1,4-dihydronicotinamide adenine dinucleotide and ethanol sensing

Analytica Chimica Acta
Siyu LiuHongguang Liu

Abstract

A facile chemical synthetic route was developed to prepare near-infrared fluorescent trypsin-stabilized Ag nanoclusters (Try-Ag NCs). The fluorescence emission wavelength of the produced Try-Ag NCs is tunable by simple adjusting pH value of the synthesis system, and the Try-Ag NCs offer a symmetric fluorescent excitation and emission peak. The fluorescence of Try-Ag NCs remains constant in the presence of various ions and molecules, and it can be effectively quenched by 1,4-dihydronicotinamide adenine dinucleotide (NADH) instead of its oxidized forms nicotinamide adenine dinucleotide (NAD(+)). This property enables the Try-Ag NCs to be a novel analytical platform to monitor biological reaction involved with NADH. In this work, the Try-Ag NCs was also applied to analyze ethanol based on the generation of NADH which was the product of NAD(+) and ethanol in the catalysis of alcohol dehydrogenase. And the proposed platform allowed ethanol to be determined in the range from 10 to 300 μmol/L with 5 μmol/L detection limit.

References

May 9, 2002·Journal of Biotechnology·Lo Gorton, Elena Domínguez
Apr 22, 2004·Journal of the American Chemical Society·Jeffrey T PettyRobert M Dickson
Oct 24, 2006·Chemical Society Reviews·J P Wilcoxon, B L Abrams
Nov 16, 2006·Annual Review of Physical Chemistry·Jie ZhengRobert M Dickson
Feb 23, 2008·Chemical Communications : Chem Comm·Li Shang, Shaojun Dong
Mar 19, 2008·Journal of the American Chemical Society·Chris I RichardsRobert M Dickson
Dec 5, 2008·Angewandte Chemie·Junhua YuRobert M Dickson
Jan 7, 2009·Journal of the American Chemical Society·Jianping XieJackie Y Ying
Apr 16, 2009·The Journal of Physical Chemistry. B·Kyeong Sik JinMoonhor Ree
Sep 19, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Kirsten SchubertFred Lisdat
Nov 6, 2009·Journal of the American Chemical Society·Zhikun WuRongchao Jin
Apr 20, 2010·Advanced Materials·Hangxun Xu, Kenneth S Suslick
Apr 22, 2010·Angewandte Chemie·T Udaya Bhaskara Rao, T Pradeep
May 6, 2010·Chemical Communications : Chem Comm·Jaswinder SharmaJennifer S Martinez
May 29, 2010·ACS Nano·Hangxun Xu, Kenneth S Suslick
Jul 21, 2010·Nanoscale·Rongchao Jin
Mar 24, 2011·Analytical Chemistry·Cunlan Guo, Joseph Irudayaraj
Oct 26, 2011·ACS Nano·Yanyan CuiXueyun Gao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Cell Invasion: Nanomedicine

Nanomedicine is a promising alternative for cancer detection and therapy that utilizes nanoparticles, such as liposomes. Nanoparticles can potentially target cancer cell invasion and metastasis. Discover the latest research on Cancer Cell Invasion: Nanomedicine here.