Facilitated gate setting by sequential dot plot scanning

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Susanne Günther, Susann Müller

Abstract

Microbial communities comprising thousands of unknown organisms can be studied flow cytometrically by applying just one fluorescent parameter and using scatter characteristics of cells. Resulting 2D-plots need to represent high numbers of cells to detect the many subcommunities, even rare ones that might be present in the sample. Evaluation of such data can be faulty and subjective due to the low number of parameters available for data discrimination and the high numbers of overlaying events. Here, we describe a procedure that helps to evaluate such data using facilitated gate setting by sequential dot-plot scanning.

References

Jan 6, 2010·Advances in Bioinformatics·Ali Bashashati, Ryan R Brinkman
Jun 11, 2010·The ISME Journal·Michael PesterAlexander Loy
Jun 29, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Enrico LugliAndrea Cossarizza
Jan 7, 2011·Bioinformatics·Francois RibaletE Virginia Armbrust
Dec 21, 2012·Environmental Science & Technology·Christin KochSusann Müller
Apr 10, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Christin KochSusann Müller

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Citations

Dec 5, 2016·FEMS Microbiology Ecology·Sally OttoLukas Y Wick
Dec 10, 2019·BMC Bioinformatics·Joachim LudwigSusann Müller
Oct 6, 2018·Microorganisms·Susanne GüntherChristian Wilhelm

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