Facilitated glucose and dehydroascorbate transport in plant mitochondria

Archives of Biochemistry and Biophysics
András SzarkaHan Asard

Abstract

Ascorbate, dehydroascorbate, and glucose transport was investigated in plant mitochondria and mitoplasts prepared from cultured BY2 tobacco cells. Using a rapid filtration method with radiolabeled ligands, we observed a specific glucose and dehydroascorbate transport, which was temperature and time dependent and saturable. Inhibition of mitochondrial respiration by KCN and the uncoupler 2,4-dinitrophenol did not influence the transport of the investigated compounds. Dehydroascorbate transport was inhibited by glucose and genistein, while glucose uptake was decreased upon 3-O-methyl-glucose, D-mannose, cytochalasin B or genistein addition. On the other hand, a low affinity low capacity ascorbate transport was found. Oxidizing agents (potassium ferricyanide or ascorbate oxidase) increased ascorbate uptake. The results demonstrate the presence of dehydroascorbate and glucose transport in plant mitochondria and suggest that it is mediated by the same or closely related transporter(s).

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Citations

Feb 26, 2009·Antioxidants & Redox Signaling·Christine H Foyer, Graham Noctor
Dec 25, 2012·Antioxidants & Redox Signaling·András SzarkaHan Asard
Feb 29, 2008·Plant Physiology·Laura ZsigmondLászló Szabados
Mar 24, 2007·FEBS Letters·Michael Büttner
Jun 11, 2009·British Journal of Pharmacology·J MandlG Bánhegyi
Aug 15, 2012·Biotechnology Journal·Eduardo Cruz-RusVictoriano Valpuesta
Jun 8, 2014·Biochimica Et Biophysica Acta·Gábor BánhegyiAndrás Szarka
Oct 15, 2013·Orvosi hetilap·András Szarka, Tamás Lőrincz
Dec 3, 2014·Journal of Theoretical Biology·András Szarka, Tibor Balogh
Jul 13, 2017·Antioxidants & Redox Signaling·Orazio CantoniMara Fiorani
May 19, 2012·International Journal of Molecular Sciences·András SzarkaGábor Bánhegyi
Aug 23, 2017·International Journal of Molecular Sciences·Alessandra GamberucciMarina Colombi
Oct 5, 2017·Antioxidants & Redox Signaling·Szilvia Z TóthAndrás Szarka
Dec 1, 2014·Botanical Studies·Jelli Venkatesh, Se Won Park
Oct 1, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sagan KCDavid W Golde
Feb 29, 2020·Journal of Experimental Botany·Haiyan DingShengchun Li
May 12, 2017·Frontiers in Plant Science·Nudrat A AkramMuhammad Ashraf
Jul 13, 2018·Frontiers in Molecular Biosciences·Vito ScaleraAnnamaria Tonazzi

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