Facilitated protein aggregation. Effects of calcium on the chaperone and anti-chaperone activity of protein disulfide-isomerase.

The Journal of Biological Chemistry
T P PrimmHiram F Gilbert

Abstract

Protein disulfide-isomerase (PDI) catalyzes the formation and isomerization of disulfides during oxidative protein folding in the eukaryotic endoplasmic reticulum. At high concentrations, it also serves as a chaperone and inhibits aggregation. However, at lower concentrations, PDI can display the unusual ability to facilitate aggregation, termed anti-chaperone activity (Puig, A., and Gilbert, H. F. (1994) J. Biol. Chem. 269, 7764-7771). Under reducing conditions (10 mM dithiothreitol) and at a low concentration (0.1-0. 3 microM) relative to the unfolded protein substrate, PDI facilitates aggregation of alcohol dehydrogenase (11 microM) that has been denatured thermally or chemically. But at higher concentrations (>0.8 microM), PDI inhibits aggregation under the same conditions. With denatured citrate synthase, PDI does not facilitate aggregation, but higher concentrations do inhibit aggregation. Anti-chaperone behavior is associated with the appearance of both PDI and substrate proteins in insoluble complexes, while chaperone behavior results in the formation of large (>500 kDa) but soluble complexes that contain both proteins. Physiological concentrations of calcium and magnesium specifically increase the apparent rate of PDI-...Continue Reading

References

Apr 1, 1992·Protein Science : a Publication of the Protein Society·W ZhiP A Srere
Jan 1, 1992·Annual Review of Biophysics and Biomolecular Structure·F U HartlW Neupert
Jan 2, 1992·Nature·M J Gething, J Sambrook
Apr 1, 1991·Protein Expression and Purification·H F GilbertJ W Harper
Mar 1, 1990·Biochimica Et Biophysica Acta·S M WestN C Price
May 8, 1969·Biochemical and Biophysical Research Communications·D J Cannon, R H McKay
Jan 1, 1984·Methods in Enzymology·D A HillsonR B Freedman
Jul 15, 1995·European Journal of Biochemistry·J L Song, C C Wang
Feb 1, 1994·Protein Expression and Purification·R Noiva
Aug 1, 1994·Trends in Biochemical Sciences·R B FreedmanM F Tuite
Jul 15, 1994·Biochemical and Biophysical Research Communications·D LebecheB Kaminer
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J KingA Mitraki
Feb 2, 1996·The Journal of Biological Chemistry·R GuthapfelE Quéméneur
Nov 1, 1995·Trends in Biochemical Sciences·P J ThomasP L Pedersen

❮ Previous
Next ❯

Citations

Jul 22, 2008·Biotechnology and Bioengineering·M MoutiezE Quéméneur
Oct 20, 2006·Applied Microbiology and Biotechnology·Leonardo M DamascenoCarl A Batt
May 26, 2004·Biochimica Et Biophysica Acta·Bonney Wilkinson, Hiram F Gilbert
Mar 10, 2004·The International Journal of Biochemistry & Cell Biology·Zhenyu GuHai-Meng Zhou
Aug 2, 2002·European Journal of Pharmacology·Alexej Verkhratsky, Ole H Petersen
Jul 14, 2001·Biochimica Et Biophysica Acta·S KatiyarW J Lennarz
Jun 29, 2000·Trends in Biochemical Sciences·E F Corbett, M Michalak
Nov 14, 2002·Biochimica Et Biophysica Acta·Luis J del VallePere Garriga
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·V SiderakiH F Gilbert
Apr 4, 2009·PLoS Pathogens·Stephanie Abromaitis, Richard S Stephens
Dec 14, 2001·Biochemical and Biophysical Research Communications·T HoribeM Kikuchi
Jan 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·R ChandrashekarK Mehta
May 5, 1999·Bioscience, Biotechnology, and Biochemistry·R UradeM Kito
Aug 22, 2014·AAPS PharmSciTech·Ahmad FazeliMohammad Reza Fazeli
Aug 4, 2015·Molecular Microbiology·Karthika KarunakaranThomas Rudel
Sep 15, 2004·Journal of Chemical Neuroanatomy·Xiaohua ShenRandal J Kaufman
Mar 2, 2006·Biochimica Et Biophysica Acta·Kazuyoshi AdoYoshihiro Taniguchi
Mar 12, 2010·Biochimica Et Biophysica Acta·Alexandra Stolz, Dieter H Wolf
Jun 9, 2015·Frontiers in Cell and Developmental Biology·Sonam Parakh, Julie D Atkin
Dec 22, 1999·Seminars in Cell & Developmental Biology·R Noiva
Dec 22, 1999·Seminars in Cell & Developmental Biology·S R Lamandé, J F Bateman
Jun 29, 2004·Biochemical and Biophysical Research Communications·Taiji KimuraMasakazu Kikuchi
Dec 31, 1997·The Journal of Biological Chemistry·H F Gilbert

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.