Facilitating tumor spheroid-based bioassays and in vitro blood vessel modeling via bioinspired self-formation microstructure devices

Lab on a Chip
Ching-Te KuoHsinyu Lee

Abstract

Non-planar microstructure-based tissue culture devices have emerged as powerful tools to mimic in vivo physiological microenvironments in a wide range of medical applications. Here we report a spontaneous aqueous molding approach - inspired by Stenocara gracilipes beetles - to rapidly fabricate non-planar microstructure devices for facilitating tissue-based bioassays. The device fabrication is determined from the self-assembled liquid morphology, which is induced by condensation or guided by surface tension. Through experiments and modeling, we reveal that the molding mainly comprises two typical circular and striped domains, highlighting versatile applications for bioengineering. In addition, the molding characteristic is dependent on the geometry of the patterned wetting surfaces, the working volume of the liquid, and the interaction between the liquid and the substrate. The theoretical model, based on the geometry of the patterned liquid, is highly consistent with experimental data. We also demonstrate that our approach can facilitate the culturing of tumor spheroids incorporated with biomimic nano-cilia, rapid high-throughput drug screening, tumor spheroid migration assay, and in vitro modeling of blood vessels. Remarkably,...Continue Reading

References

Apr 20, 1999·The Journal of Clinical Investigation·S LiS Chien
Jul 17, 2001·Biochemical and Biophysical Research Communications·P P HsuS Chien
Dec 17, 2002·Journal of Applied Physiology·Yasushi ShikataJoe G N Garcia
Aug 28, 2007·Cell·Kenneth M Yamada, Edna Cukierman
Oct 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ratmir DerdaGeorge M Whitesides
Mar 30, 2010·Nature Materials·Kuang-Han ChuEvelyn N Wang
Sep 4, 2012·Mutation Research·Nina KramerHelmut Dolznig
Dec 12, 2012·Clinical Chemistry·Nemanja D MarjanovicChristine L Chaffer
Jun 28, 2014·Cancer Research·Andrew D Wong, Peter C Searson
Feb 17, 2015·Lab on a Chip·Yuzhen GuoYanlin Song
Sep 9, 2015·Journal of Cell Science·Hiroshi YukiuraJunken Aoki
Sep 30, 2015·Scientific Reports·Andrea WeissPatrycja Nowak-Sliwinska
Mar 19, 2016·Advanced Materials·Yiwei LiBi-Feng Liu
Jun 28, 2016·ACS Nano·Inhee ChoiLuke P Lee
Jun 30, 2016·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Philip S Brown, Bharat Bhushan
Jun 24, 2017·Advanced Materials·Peidi XuQionglin Liang
Feb 19, 2016·Materials·Sera ShinTaeyoon Lee
Sep 2, 2017·Lab on a Chip·Yongha Hwang, Rob N Candler

❮ Previous
Next ❯

Citations

Mar 29, 2019·Lab on a Chip·Bin ChenFeng Guo
Feb 19, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Changmin ShaoYuanjin Zhao

❮ Previous
Next ❯

Methods Mentioned

BETA
biosensing

Software Mentioned

CompuSYN
COMSOL
ParaStamp
Matlab
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.