PMID: 7580873Aug 1, 1995Paper

Facilitation and inhibition of G-protein regulated protein secretion by melatonin

Neurochemistry International
M Bubis, N Zisapel

Abstract

Melatonin has been found to inhibit or enhance the constitutive secretion of proteins from the cultured melanoma cells at nanomolar concentrations (0.5-10 nM), in a dose dependent manner. The amplitude and direction of the response were found to depend on cell density: melatonin inhibited the release early after plating or at low cell density, but facilitated the release later on, or at high cell density. To elucidate the involvement of G-proteins in these responses, the effects of guanosine 5'-O-(3-thiotriphosphate) (GTP tau S; which was introduced into the cells during the process of permeabilization and resealing with ATP), aluminum fluoride, pertussis and cholera toxins on protein secretion from the cells were assessed in the absence and presence of melatonin. At low cell density, melatonin inhibited release, but paradoxically enhanced it when GTP hydrolysis was blocked (by GTP tau S or cholera toxin treatment). Aluminum fluoride and melatonin inhibited protein release in the absence or presence of GTP tau S. At high cell density, melatonin facilitated the release and so did GTP tau S, aluminum fluoride, their combination, and cholera toxin treatment. However, in the presence of the combination of GTP tau S, aluminium fluor...Continue Reading

References

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Dec 1, 1993·Melanoma Research·R A HeltonM A Kane

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Citations

May 13, 1999·Biology of the Cell·M Bubis, N Zisapel
Oct 30, 1996·Molecular and Cellular Endocrinology·M BubisN Zisapel
Jun 2, 1998·Molecular and Cellular Endocrinology·M Bubis, N Zisapel
May 26, 1999·Journal of Pineal Research·E GiladN Zisapel
Oct 29, 2014·Journal of Toxicology·Christopher A ShawRobert M Davidson
Oct 20, 2018·Frontiers in Microbiology·Xian ChenFengquan Liu

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