Facilitation of axon outgrowth via a Wnt5a-CaMKK-CaMKIα pathway during neuronal polarization

Molecular Brain
Shin-ichiro HoriganeHaruhiko Bito

Abstract

Wnt5a, originally identified as a guidance cue for commissural axons, activates a non-canonical pathway critical for cortical axonal morphogenesis. The molecular signaling cascade underlying this event remains obscure. Through Ca(2+) imaging in acute embryonic cortical slices, we tested if radially migrating cortical excitatory neurons that already bore primitive axons were sensitive to Wnt5a. While Wnt5a only evoked brief Ca(2+) transients in immature neurons present in the intermediate zone (IZ), Wnt5a-induced Ca(2+) oscillations were sustained in neurons that migrated out to the cortical plate (CP). We wondered whether this early Wnt5a-Ca(2+) signaling during neuronal polarization has a morphogenetic consequence. During transition from round to polarized shape, Wnt5a administration to immature cultured cortical neurons specifically promoted axonal, but not dendritic, outgrowth. Pharmacological and genetic inhibition of the CaMKK-CaMKIα pathway abolished Wnt5a-induced axonal elongation, and rescue of CaMKIα in CaMKIα-knockdown neurons restored Wnt5a-mediated axon outgrowth. This study suggests that Wnt5a activates Ca(2+) signaling during a neuronal morphogenetic time window when axon outgrowth is critically facilitated. Furth...Continue Reading

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Citations

Jun 21, 2017·Journal of Proteomics·Anup D SharmaDonald S Sakaguchi
Feb 13, 2019·Journal of Biochemistry·Shin-Ichiro HoriganeSayaka Takemoto-Kimura
Mar 16, 2017·Journal of Neurochemistry·Sayaka Takemoto-KimuraHaruhiko Bito
Nov 29, 2020·Cellular and Molecular Life Sciences : CMLS·Valeria KalteziotiPanagiotis K Politis

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